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01-04-2016 | Original Contribution

Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer

Authors: Peter Kubatka, Andrea Kapinová, Martin Kello, Peter Kruzliak, Karol Kajo, Desanka Výbohová, Silvia Mahmood, Radovan Murin, Tischlerová Viera, Ján Mojžiš, Anthony Zulli, Martin Péč, Marián Adamkov, Monika Kassayová, Bianka Bojková, Nadežda Stollárová, Dušan Dobrota

Published in: European Journal of Nutrition | Issue 3/2016

Abstract

Purpose

Fruit and vegetable intake is inversely correlated with cancer; thus, it is proposed that an extract of phytochemicals as present in whole fruits, vegetables, or grains may have anti-carcinogenic properties. Thus, the anti-tumour effects of fruit peel polyphenols (Flavin7) in the chemoprevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis in female rats were evaluated.

Methods

Lyophilized substance of Flavin7 (F7) was administered at two concentrations of 0.3 and 3 % through diet. The experiment was terminated 14 weeks after carcinogen administration, and mammary tumours were removed and prepared for histopathological and immunohistochemical analysis. In addition, using an in vitro cytotoxicity assay, apoptosis and proliferation after F7 treatment in human breast adenocarcinoma (MCF-7) cells were performed.

Results

High-dose F7 suppressed tumour frequency by 58 % (P < 0.001), tumour incidence by 24 % (P < 0.05), and lengthened latency by 8 days (P > 0.05) in comparison with the control rats, whereas lower dose of F7 was less effective. Histopathological analysis of tumours showed significant decrease in the ratio of high-/low-grade carcinomas after high-dose F7 treatment. Immunohistochemical analysis of rat carcinoma cells in vivo found a significant increase in caspase-3 expression and significant decrease in Bcl-2, Ki67, and VEGFR-2 expression in the high-dose group. Both doses demonstrated significant positive effects on plasma lipid metabolism in rats. F7 significantly decreased survival of MCF-7 cells in vitro in MTT assay by dose- and time-dependent manner compared to control. F7 prevented cell cycle progression by significant enrichment in G1 cell populations. Incubation with F7 showed significant increase in the percentage of annexin V-/PI-positive MCF-7 cells and DNA fragmentation.

Conclusions

Our results reveal a substantial tumour-suppressive effect of F7 in the breast cancer model. We propose that the effects of phytochemicals present in this fruit extract are responsible for observed potent anti-cancer activities.

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Title: Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer
Authors: Peter Kubatka
Andrea Kapinová
Martin Kello
Peter Kruzliak
Karol Kajo
Desanka Výbohová
Silvia Mahmood
Radovan Murin
Tischlerová Viera
Ján Mojžiš
Anthony Zulli
Martin Péč
Marián Adamkov
Monika Kassayová
Bianka Bojková
Nadežda Stollárová
Dušan Dobrota
Publication date: 01-04-2016
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 3/2016
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-015-0910-5

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