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European Journal of Nutrition

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01-04-2013 | Original Contribution

Fate of dietary phytosteryl/-stanyl esters: analysis of individual intact esters in human feces

Authors: Tim Lubinus, Andreas Barnsteiner, Thomas Skurk, Hans Hauner, Karl-Heinz Engel

Published in: European Journal of Nutrition | Issue 3/2013

Abstract

Purpose

The objective was to investigate the metabolic fate of phytosteryl/-stanyl fatty acid and ferulic acid esters upon consumption by healthy humans.

Methods

A capillary gas chromatographic methodology was employed to follow a randomized, single-blind three group crossover clinical trial and to quantify simultaneously individual intact esters, liberated phytosterols/-stanols and their metabolites in feces. Skimmed milk drinking yogurts enriched with complex mixtures of phytosteryl/-stanyl fatty acid esters and ferulates, respectively, were employed as food carriers.

Results

On average, 73 % of total plant stanyl fatty acid esters and 80 % of total plant steryl fatty acid esters were hydrolyzed. Among the individuals, the hydrolysis rates ranged from 40 to 96 %. In addition, there were subject-dependent discrepancies between the amounts of phytosterols/-stanols actually determined in the feces and the calculated hydrolysis rates. On average, 69 % of the amounts of sterols/stanols expected from the amounts of remaining intact esters were found.

Conclusions

The study revealed large interindividual variability regarding the recoveries of dietary phytosteryl/-stanyl esters upon gastrointestinal passage in healthy humans. Nevertheless, there was a significant impact of the acid moiety (oleate = linoleate = linolenate > eicosanoate > palmitate > ferulate) on the hydrolysis rates; the influence of the phytosterol/-stanol moiety was less pronounced.

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Title: Fate of dietary phytosteryl/-stanyl esters: analysis of individual intact esters in human feces
Authors: Tim Lubinus
Andreas Barnsteiner
Thomas Skurk
Hans Hauner
Karl-Heinz Engel
Publication date: 01-04-2013
Publisher: Springer-Verlag
Published in: European Journal of Nutrition / Issue 3/2013
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-012-0407-4

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Purpose

Methods

Results

Conclusions

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