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European Journal of Nutrition
Published in: European Journal of Nutrition 2/2013

Open Access 01-03-2013 | Original Contribution

Oil palm phenolics attenuate changes caused by an atherogenic diet in mice

Authors: Soon-Sen Leow, Shamala Devi Sekaran, Kalyana Sundram, YewAi Tan, Ravigadevi Sambanthamurthi

Published in: European Journal of Nutrition | Issue 2/2013

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Abstract

Background

Water-soluble phenolics from the oil palm possess significant biological properties.

Purpose

In this study, we aimed to discover the role of oil palm phenolics (OPP) in influencing the gene expression changes caused by an atherogenic diet in mice.

Methods

We fed mice with either a low-fat normal diet (14.6 % kcal/kcal fat) with distilled water, or a high-fat atherogenic diet (40.5 % kcal/kcal fat) containing cholesterol. The latter group was given either distilled water or OPP. We harvested major organs such as livers, spleens and hearts for microarray gene expression profiling analysis. We determined how OPP changed the gene expression profiles caused by the atherogenic diet. In addition to gene expression studies, we carried out physiological observations, blood hematology as well as clinical biochemistry, cytokine profiling and antioxidant assays on their blood sera.

Results

Using Illumina microarrays, we found that the atherogenic diet caused oxidative stress, inflammation and increased turnover of metabolites and cells in the liver, spleen and heart. In contrast, OPP showed signs of attenuating these effects. The extract increased unfolded protein response in the liver, attenuated antigen presentation and processing in the spleen and up-regulated antioxidant genes in the heart. Real-time quantitative reverse transcription-polymerase chain reaction validated the microarray gene expression fold changes observed. Serum cytokine profiling showed that OPP attenuated inflammation by modulating the Th1/Th2 axis toward the latter. OPP also increased serum antioxidant activity to normal levels.

Conclusion

This study suggests that OPP may possibly attenuate atherosclerosis and other forms of cardiovascular disease.
Appendix
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Literature
1.
Liu RH (2003) Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr 78:517S–520S
2.
Noguchi N, Niki E (2000) Phenolic antioxidants: a rationale for design and evaluation of novel antioxidant drug for atherosclerosis. Free Radic Biol Med 28:1538–1546CrossRef
3.
Hu FB, Willett WC (2002) Optimal diets for prevention of coronary heart disease. JAMA 288:2569–2578CrossRef
4.
Hu FB (2003) Plant-based foods and prevention of cardiovascular disease: an overview. Am J Clin Nutr 78:544S–551S
5.
Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA (2001) Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 74:418–425
6.
Arts IC, Hollman PC (2005) Polyphenols and disease risk in epidemiologic studies. Am J Clin Nutr 81:317S–325S
7.
Halliwell B (2008) Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies? Arch Biochem Biophys 476:107–112CrossRef
8.
Hollman PC, Cassidy A, Comte B, Heinonen M, Richelle M, Richling E, Serafini M, Scalbert A, Sies H, Vidry S (2011) The biological relevance of direct antioxidant effects of polyphenols for cardiovascular health in humans is not established. J Nutr 141:989S–1009SCrossRef
9.
Jiang F, Dusting GJ (2003) Natural phenolic compounds as cardiovascular therapeutics: potential role of their antiinflammatory effects. Curr Vasc Pharmacol 1:135–156CrossRef
10.
Kirk EA, Sutherland P, Wang SA, Chait A, LeBoeuf RC (1998) Dietary isoflavones reduce plasma cholesterol and atherosclerosis in C57BL/6 mice but not LDL receptor-deficient mice. J Nutr 128:954–959
11.
Aviram M, Dornfeld L (2001) Pomegranate juice consumption inhibits serum angiotensin converting enzyme activity and reduces systolic blood pressure. Atherosclerosis 158:195–198CrossRef
12.
Aviram M, Dornfeld L, Rosenblat M, Volkova N, Kaplan M, Coleman R, Hayek T, Presser D, Fuhrman B (2000) Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. Am J Clin Nutr 71:1062–1076
13.
Fuhrman B, Rosenblat M, Hayek T, Coleman R, Aviram M (2000) Ginger extract consumption reduces plasma cholesterol, inhibits LDL oxidation and attenuates development of atherosclerosis in atherosclerotic, apolipoprotein E-deficient mice. J Nutr 130:1124–1131
14.
Ghayur MN, Gilani AH, Afridi MB, Houghton PJ (2005) Cardiovascular effects of ginger aqueous extract and its phenolic constituents are mediated through multiple pathways. Vascul Pharmacol 43:234–241CrossRef
15.
Stocker R, O’Halloran RA (2004) Dealcoholized red wine decreases atherosclerosis in apolipoprotein E gene-deficient mice independently of inhibition of lipid peroxidation in the artery wall. Am J Clin Nutr 79:123–130
16.
Waddington E, Puddey IB, Croft KD (2004) Red wine polyphenolic compounds inhibit atherosclerosis in apolipoprotein E-deficient mice independently of effects on lipid peroxidation. Am J Clin Nutr 79:54–61
17.
Gonzalez-Santiago M, Martin-Bautista E, Carrero JJ, Fonolla J, Baro L, Bartolome MV, Gil-Loyzaga P, Lopez-Huertas E (2006) One-month administration of hydroxytyrosol, a phenolic antioxidant present in olive oil, to hyperlipemic rabbits improves blood lipid profile, antioxidant status and reduces atherosclerosis development. Atherosclerosis 188:35–42CrossRef
18.
Morton LW, Abu-Amsha Caccetta R, Puddey IB, Croft KD (2000) Chemistry and biological effects of dietary phenolic compounds: relevance to cardiovascular disease. Clin Exp Pharmacol Physiol 27:152–159CrossRef
19.
Sambanthamurthi R, Sundram K, Tan YA (2000) Chemistry and biochemistry of palm oil. Prog Lipid Res 39:507–558CrossRef
20.
Sundram K, Sambanthamurthi R, Tan YA (2003) Palm fruit chemistry and nutrition. Asia Pac J Clin Nutr 12:355–362
21.
Tan YA, Sambanthamurthi R, Sundram K, Wahid MB (2007) Valorisation of palm by-products as functional components. Eur J Lipid Sci Technol 109:380–393CrossRef
22.
Sambanthamurthi R, Tan YA, Sundram K (inventors) Malaysian Palm Oil Board (assignee) (2008) Treatment of vegetation liquors derived from oil-bearing fruit. United States patent US 7387802 B2
23.
Sambandan TG, Rha CK, Sinskey AJ, Sambanthamurthi R, Tan YA, Sundram K, Wahid MB (inventors) Malaysian Palm Oil Board (assignee) (2010) Composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid and their derivatives and method of preparation thereof. World patent application publication WO 2010137943
24.
Sambanthamurthi R, Tan YA, Sundram K, Abeywardena M, Sambandan TG, Rha C, Sinskey AJ, Subramaniam K, Leow SS, Hayes KC, Basri Wahid M (2011) Oil palm vegetation liquor: a new source of phenolic bioactives. Br J Nutr 106:1655–1663CrossRef
25.
Sambanthamurthi R, Tan YA, Sundram K, Hayes KC, Abeywardena M, Leow SS, Sekaran SD, Sambandan TG, Rha C, Sinskey AJ, Subramaniam K, Fairus S, Basri Wahid M (2011) Positive outcomes of oil palm phenolics on degenerative diseases in animal models. Br J Nutr 106:1664–1675CrossRef
26.
Sekaran SD, Leow SS, Abobaker N, Tee KK, Sundram K, Sambanthamurthi R, Wahid MB (2010) Effects of oil palm phenolics on tumor cells in vitro and in vivo. Afr J Food Sci 4:495–502
27.
Leow SS, Sekaran SD, Sundram K, Tan YA, Sambanthamurthi R (2011) Differential transcriptomic profiles effected by oil palm phenolics indicate novel health outcomes. BMC Genomics 12:432CrossRef
28.
Edgar R, Domrachev M, Lash AE (2002) Gene expression omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res 30:207–210CrossRef
29.
Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N, Braisted J, Klapa M, Currier T, Thiagarajan M, Sturn A, Snuffin M, Rezantsev A, Popov D, Ryltsov A, Kostukovich E, Borisovsky I, Liu Z, Vinsavich A, Trush V, Quackenbush J (2003) TM4: a free, open-source system for microarray data management and analysis. Biotechniques 34:374–378
30.
Novak JP, Miller MC 3rd, Bell DA (2006) Variation in fiberoptic bead-based oligonucleotide microarrays: dispersion characteristics among hybridization and biological replicate samples. Biol Direct 1:18CrossRef
31.
Dahlquist KD, Salomonis N, Vranizan K, Lawlor SC, Conklin BR (2002) GenMAPP, a new tool for viewing and analyzing microarray data on biological pathways. Nat Genet 31:19–20CrossRef
32.
Doniger SW, Salomonis N, Dahlquist KD, Vranizan K, Lawlor SC, Conklin BR (2003) MAPPFinder: using gene ontology and GenMAPP to create a global gene-expression profile from microarray data. Genome Biol 4:R7CrossRef
33.
Bustin SA, Nolan T (2004) Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J Biomol Technol 15:155–166
34.
Nolan T, Hands RE, Bustin SA (2006) Quantification of mRNA using real-time RT-PCR. Nat Protoc 1:1559–1582CrossRef
35.
Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8:R19CrossRef
36.
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:RESEARCH0034
37.
Gao X, Ohlander M, Jeppsson N, Bjork L, Trajkovski V (2000) Changes in antioxidant effects and their relationship to phytonutrients in fruits of sea buckthorn (Hippophae rhamnoides L.) during maturation. J Agric Food Chem 48:1485–1490CrossRef
38.
Maskarinec G, Chan CL, Meng L, Franke AA, Cooney RV (1999) Exploring the feasibility and effects of a high-fruit and -vegetable diet in healthy women. Cancer Epidemiol Biomarkers Prev 8:919–924
39.
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76CrossRef
40.
Manthey JA (2004) Fractionation of orange peel phenols in ultrafiltered molasses and mass balance studies of their antioxidant levels. J Agric Food Chem 52:7586–7592CrossRef
41.
Truong VD, McFeeters RF, Thompson RT, Dean LL, Shofran B (2007) Phenolic acid content and composition in leaves and roots of common commercial sweetpotato (Ipomea batatas L.) cultivars in the United States. J Food Sci 72:C343–C349CrossRef
42.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237CrossRef
43.
Reagan-Shaw S, Nihal M, Ahmad N (2008) Dose translation from animal to human studies revisited. Faseb J 22:659–661CrossRef
44.
Kleemann R, Verschuren L, van Erk MJ, Nikolsky Y, Cnubben NH, Verheij ER, Smilde AK, Hendriks HF, Zadelaar S, Smith GJ, Kaznacheev V, Nikolskaya T, Melnikov A, Hurt-Camejo E, van der Greef J, van Ommen B, Kooistra T (2007) Atherosclerosis and liver inflammation induced by increased dietary cholesterol intake: a combined transcriptomics and metabolomics analysis. Genome Biol 8:R200CrossRef
45.
Kreeft AJ, Moen CJ, Porter G, Kasanmoentalib S, Sverdlov R, van Gorp PJ, Havekes LM, Frants RR, Hofker MH (2005) Genomic analysis of the response of mouse models to high-fat feeding shows a major role of nuclear receptors in the simultaneous regulation of lipid and inflammatory genes. Atherosclerosis 182:249–257CrossRef
46.
Recinos A 3rd, Carr BK, Bartos DB, Boldogh I, Carmical JR, Belalcazar LM, Brasier AR (2004) Liver gene expression associated with diet and lesion development in atherosclerosis-prone mice: induction of components of alternative complement pathway. Physiol Genomics 19:131–142CrossRef
47.
Maxwell KN, Soccio RE, Duncan EM, Sehayek E, Breslow JL (2003) Novel putative SREBP and LXR target genes identified by microarray analysis in liver of cholesterol-fed mice. J Lipid Res 44:2109–2119CrossRef
48.
Hansson GK (2001) Immune mechanisms in atherosclerosis. Arterioscler Thromb Vasc Biol 21:1876–1890CrossRef
49.
Hansson GK, Libby P, Schonbeck U, Yan ZQ (2002) Innate and adaptive immunity in the pathogenesis of atherosclerosis. Circ Res 91:281–291CrossRef
50.
Ludewig B, Krebs P, Scandella E (2004) Immunopathogenesis of atherosclerosis. J Leukoc Biol 76:300–306CrossRef
51.
Levy DE, Lee CK (2002) What does Stat3 do? J Clin Invest 109:1143–1148
52.
Sen CK, Packer L (1996) Antioxidant and redox regulation of gene transcription. Faseb J 10:709–720
53.
Khachigian LM, Fahmy RG, Zhang G, Bobryshev YV, Kaniaros A (2002) c-Jun regulates vascular smooth muscle cell growth and neointima formation after arterial injury. Inhibition by a novel DNA enzyme targeting c-Jun. J Biol Chem 277:22985–22991CrossRef
54.
Knight RJ, Buxton DB (1996) Stimulation of c-Jun kinase and mitogen-activated protein kinase by ischemia and reperfusion in the perfused rat heart. Biochem Biophys Res Commun 218:83–88CrossRef
55.
Ueyama T, Umemoto S, Senba E (1996) Immobilization stress induces c-fos and c-jun immediate early genes expression in the heart. Life Sci 59:339–347CrossRef
56.
Cook SA, Sugden PH, Clerk A (1999) Activation of c-Jun N-terminal kinases and p38-mitogen-activated protein kinases in human heart failure secondary to ischaemic heart disease. J Mol Cell Cardiol 31:1429–1434CrossRef
57.
Sasaki H, Galang N, Maulik N (1999) Redox regulation of NF-kappaB and AP-1 in ischemic reperfused heart. Antioxid Redox Signal 1:317–324CrossRef
58.
Fan H, Sun B, Gu Q, Lafond-Walker A, Cao S, Becker LC (2002) Oxygen radicals trigger activation of NF-kappaB and AP-1 and upregulation of ICAM-1 in reperfused canine heart. Am J Physiol Heart Circ Physiol 282:H1778–H1786
59.
Freire G, Ocampo C, Ilbawi N, Griffin AJ, Gupta M (2007) Overt expression of AP-1 reduces alpha myosin heavy chain expression and contributes to heart failure from chronic volume overload. J Mol Cell Cardiol 43:465–478CrossRef
60.
Grainger DJ (2004) Transforming growth factor beta and atherosclerosis: so far, so good for the protective cytokine hypothesis. Arterioscler Thromb Vasc Biol 24:399–404CrossRef
61.
Grainger DJ (2007) TGF-beta and atherosclerosis in man. Cardiovasc Res 74:213–222CrossRef
62.
Tabibiazar R, Wagner RA, Ashley EA, King JY, Ferrara R, Spin JM, Sanan DA, Narasimhan B, Tibshirani R, Tsao PS, Efron B, Quertermous T (2005) Signature patterns of gene expression in mouse atherosclerosis and their correlation to human coronary disease. Physiol Genomics 22:213–226CrossRef
63.
Sharma S, Sharma R (2001) Biochemical evaluation of lipid and oxidative stress status in relation to high fat-high antioxidant diets. Indian J Exp Biol 39:1180–1183
64.
Yang R, Le G, Li A, Zheng J, Shi Y (2006) Effect of antioxidant capacity on blood lipid metabolism and lipoprotein lipase activity of rats fed a high-fat diet. Nutrition 22:1185–1191CrossRef
65.
Cardona F, Tunez I, Tasset I, Garrido-Sanchez L, Collantes E, Tinahones FJ (2008) Circulating antioxidant defences are decreased in healthy people after a high-fat meal. Br J Nutr 100:312–316CrossRef
66.
Wojcik C, Rowicka M, Kudlicki A, Nowis D, McConnell E, Kujawa M, DeMartino GN (2006) Valosin-containing protein (p97) is a regulator of endoplasmic reticulum stress and of the degradation of N-end rule and ubiquitin-fusion degradation pathway substrates in mammalian cells. Mol Biol Cell 17:4606–4618CrossRef
67.
Lee MH, Hyun DH, Jenner P, Halliwell B (2001) Effect of proteasome inhibition on cellular oxidative damage, antioxidant defences and nitric oxide production. J Neurochem 78:32–41CrossRef
68.
Weindruch R, Kayo T, Lee CK, Prolla TA (2001) Microarray profiling of gene expression in aging and its alteration by caloric restriction in mice. J Nutr 131:918S–923S
69.
Ross R (1999) Atherosclerosis–an inflammatory disease. N Engl J Med 340:115–126CrossRef
70.
Kantengwa S, Jornot L, Devenoges C, Nicod LP (2003) Superoxide anions induce the maturation of human dendritic cells. Am J Respir Crit Care Med 167:431–437CrossRef
71.
Ma Q, Kinneer K (2002) Chemoprotection by phenolic antioxidants. Inhibition of tumor necrosis factor alpha induction in macrophages. J Biol Chem 277:2477–2484CrossRef
72.
Moskaug JO, Carlsen H, Myhrstad MC, Blomhoff R (2005) Polyphenols and glutathione synthesis regulation. Am J Clin Nutr 81:277S–283S
73.
Hansson GK (2005) Inflammation, atherosclerosis, and coronary artery disease. N Engl J Med 352:1685–1695CrossRef
74.
Kleemann R, Zadelaar S, Kooistra T (2008) Cytokines and atherosclerosis: a comprehensive review of studies in mice. Cardiovasc Res 79:360–376CrossRef
75.
Abbas AK, Lichtman AH (2005) Cellular and molecular immunology. Elsevier, Philadelphia
76.
Lee TS, Yen HC, Pan CC, Chau LY (1999) The role of interleukin 12 in the development of atherosclerosis in ApoE-deficient mice. Arterioscler Thromb Vasc Biol 19:734–742CrossRef
77.
Davenport P, Tipping PG (2003) The role of interleukin-4 and interleukin-12 in the progression of atherosclerosis in apolipoprotein E-deficient mice. Am J Pathol 163:1117–1125CrossRef
78.
Hasko G, Szabo C (1999) IL-12 as a therapeutic target for pharmacological modulation in immune-mediated and inflammatory diseases: regulation of T helper 1/T helper 2 responses. Br J Pharmacol 127:1295–1304CrossRef
79.
Patel NS, Paris D, Mathura V, Quadros AN, Crawford FC, Mullan MJ (2005) Inflammatory cytokine levels correlate with amyloid load in transgenic mouse models of Alzheimer’s disease. J Neuroinflamm 2:9CrossRef
80.
O’Neill TP (1997) Apolipoprotein E-deficient mouse model of human atherosclerosis. Toxicol Pathol 25:20–21CrossRef
81.
Tabibiazar R, Wagner RA, Spin JM, Ashley EA, Narasimhan B, Rubin EM, Efron B, Tsao PS, Tibshirani R, Quertermous T (2005) Mouse strain-specific differences in vascular wall gene expression and their relationship to vascular disease. Arterioscler Thromb Vasc Biol 25:302–308CrossRef
Metadata
Title
Oil palm phenolics attenuate changes caused by an atherogenic diet in mice
Authors
Soon-Sen Leow
Shamala Devi Sekaran
Kalyana Sundram
YewAi Tan
Ravigadevi Sambanthamurthi
Publication date
01-03-2013
Publisher
Springer-Verlag
Published in
European Journal of Nutrition / Issue 2/2013
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-012-0346-0

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