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European Journal of Nutrition

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01-08-2009 | Original Contribution

Lycopene isomerisation and storage in an in vitro model of murine hepatic stellate cells

Authors: Anderson J. Teodoro, Daniel Perrone, Renata B. Martucci, Radovan Borojevic

Published in: European Journal of Nutrition | Issue 5/2009

Abstract

Background

Lycopene is a carotenoid whose biological activities and protective effect on prostate and breast cancer have been described, but little is known on its extra-intestinal metabolism and storage. While most alimentary lycopene is in all-trans configuration, in animal and human tissues approximately half of the lycopene is in cis isoforms.

Aim of study

Our object was to monitor the capacity of storage, isomerisation, and intracellular localization of all-trans and cis lycopene in hepatic stellate cells, which are the major sites of metabolism and storage of retinoids and carotenoids in the body.

Methods

We used the GRX cell line representative of murine hepatic stellate cells, incubated with 1–30 μM lycopene in culture medium. Analysis was done by high-performance liquid chromatography.

Results

Lycopene was able to induce expression of the lipocyte phenotype and it was internalized into GRX cells. Its cellular release only occurred in presence of albumin with a rapid initial decrease of intracellular lycopene. A corresponding increase in the culture medium was observed at 24 h. All-trans, 13-cis and 9-cis lycopene isoforms were identified in all the cell compartments. The membrane fraction contained the major part of lycopene, followed by the cytoplasmic fraction, lipid droplets and nuclei. The ratio between all-trans and cis isomers was approximately 2/1 in the majority parts of cell compartments.

Conclusions

This study identified a novel hepatic cell type able to store and isomerise lycopene. Liver can contribute to the serum and tissue equilibrium of cis/trans isomers of lycopene, and to participate in storage of lycopene under high extracellular concentration such as observed after the alimentary input.

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Title: Lycopene isomerisation and storage in an in vitro model of murine hepatic stellate cells
Authors: Anderson J. Teodoro
Daniel Perrone
Renata B. Martucci
Radovan Borojevic
Publication date: 01-08-2009
Publisher: D. Steinkopff-Verlag
Published in: European Journal of Nutrition / Issue 5/2009
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-009-0001-6

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Lycopene isomerisation and storage in an in vitro model of murine hepatic stellate cells

Abstract

Background

Aim of study

Methods

Results

Conclusions

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Abstract

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Conclusions

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