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Published in: Clinical Research in Cardiology 8/2016

01-08-2016 | Original Paper

Influence of exercise training on proangiogenic TIE-2 monocytes and circulating angiogenic cells in patients with peripheral arterial disease

Authors: Jörn F. Dopheide, Philipp Geissler, Jennifer Rubrech, Amelie Trumpp, Geraldine C. Zeller, Andreas Daiber, Thomas Münzel, Markus P. Radsak, Christine Espinola-Klein

Published in: Clinical Research in Cardiology | Issue 8/2016

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Abstract

Background

Inflammation is the driving force in atherosclerosis. One central strategy in the treatment of peripheral arterial disease (PAD) is the promotion of angiogenesis. Here, proangiogenic Tie-2 expressing monocytes (TEM) and circulating angiogenic cells (CAC) play a crucial role. Exercise training (ET) is recommended in PAD patients at Fontaine stage II to promote angiogenesis.

Methods

40 patients with intermittend claudication (IC) [2 groups: supervised ET (SET) vs. non-supervised ET (nSET), each n = 20] and 20 healthy controls were included in the study. Analysis of TEM and CAC was performed from whole blood by flow-cytometry. TEM were identified via CD45, CD86, CD14, CD16 and analysed for the expression of Tie-2. CAC were identified via their expression of CD45 (CD45dim), CD34 and VEGF-R2 (CD309/KDR). Follow up was performed after mean of 7.65 ± 1.62 months.

Results

In comparison to healthy controls, we found increased proportions of CAC (p < 0.0001) and similar TEM numbers in both ET groups. At follow-up (FU) TEM poroportions increased (p < 0.001) and CAC proportions decreased (p < 0.01), but both more significantly in SET (p < 0.001) than nSET (p = 0.01). Only in SET fibrinogen levels decreased and VEGF-A increased (both p < 0.05). Finally, we found in both ET groups a significant increase in absolute walking distance but with a higher individual increase in SET (p < 0.01). TEM and CAC proportions correlated inversely with the absolute walking distance (CAC: r = −0.296, p = 0.02; TEM: r = −0.270, p = 0.04) as well as with ABI (CAC: r = −0.394, p < 0.01; TEM: r = −0.382, p < 0.01).

Conclusions

ET influences the distribution of CAC and TEM proportions. nSET, although still effective in regard to an improved walking distance, is less effective in the influence of proangiogenic cells and inflammatory burden than SET. Our results indicate SET to be a more preferential exercise form, supporting the necessity to establish more SET programs.
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Metadata
Title
Influence of exercise training on proangiogenic TIE-2 monocytes and circulating angiogenic cells in patients with peripheral arterial disease
Authors
Jörn F. Dopheide
Philipp Geissler
Jennifer Rubrech
Amelie Trumpp
Geraldine C. Zeller
Andreas Daiber
Thomas Münzel
Markus P. Radsak
Christine Espinola-Klein
Publication date
01-08-2016
Publisher
Springer Berlin Heidelberg
Published in
Clinical Research in Cardiology / Issue 8/2016
Print ISSN: 1861-0684
Electronic ISSN: 1861-0692
DOI
https://doi.org/10.1007/s00392-016-0966-0

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