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14-06-2022 | Metastasis | Original Article

Antitumor effect of polyphyllin D on liver metastases of neuroblastoma

Authors: Yasuhiro Kondo, Shunsuke Watanabe, Atsuki Naoe, Toshiyuki Takeuchi, Atsuko Niimi, Motoshi Suzuki, Naoya Asai, Seiji Okada, Tomonori Tsuchiya, Mika Murayama, Toshihiro Yasui, Mikihiro Inoue, Tatsuya Suzuki

Published in: Pediatric Surgery International | Issue 8/2022

Abstract

Purpose

We previously reported that polyphyllin D, a main component of the traditional Chinese medicinal herb Paris polyphylla, exhibited anticancer effects in vitro against human neuroblastoma cells. The aims of this investigation was to examine the presence or absence of in vivo anti-metastasis effects of polyphyllin D were to establish a liver metastasis model of neuroblastoma and to evaluate the anti-metastasis effects of polyphyllin D.

Methods

Subcutaneous and intraperitoneal tumors, and metastasis models were established in immune-deficient BALB/c nude and BALB/c Rag-2/Jak3 double-deficient (BRJ) mice using the human neuroblastoma cell lines IMR-32, LA–N-2, or NB-69. For evaluating polyphyllin D activity, we used a mouse model of liver metastasis with the IMR-32 cells line injected through the tail vein. We analyzed the livers number and area of liver tumors in of the phosphate buffer solution- and polyphyllin D-treated groups.

Results

Liver metastasis and intraperitoneal dissemination models were successfully established in immune-deficient BRJ mice using the three human neuroblastoma cell lines. In the liver metastasis, the model of IMR-32 cells, we found that polyphyllin D suppressed both the number and total area of metastatic foci the average number of metastatic foci, average focus areas, and number of cleaved caspase-3-positive cells were significantly lower in the polyphyllin D group (p = 0.016, 0.020, 0.043, respectively).

Conclusions

We developed a mouse models of neuroblastoma metastasis and demonstrated for the first time that polyphyllin D has an antitumor effect on neuroblastoma liver metastases.

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Title: Antitumor effect of polyphyllin D on liver metastases of neuroblastoma
Authors: Yasuhiro Kondo
Shunsuke Watanabe
Atsuki Naoe
Toshiyuki Takeuchi
Atsuko Niimi
Motoshi Suzuki
Naoya Asai
Seiji Okada
Tomonori Tsuchiya
Mika Murayama
Toshihiro Yasui
Mikihiro Inoue
Tatsuya Suzuki
Publication date: 14-06-2022
Publisher: Springer Berlin Heidelberg
Keywords: Metastasis
Neuroblastoma
Neuroblastoma
Published in: Pediatric Surgery International / Issue 8/2022
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-022-05146-7

At a glance: The STEP trials

Springer Medicine

Antitumor effect of polyphyllin D on liver metastases of neuroblastoma

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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