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01-01-2009 | Original Paper

Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis

Authors: Jian-Jun Zhao, Juhua Yang, Jianhong Lin, Nan Yao, Yihua Zhu, Jianlong Zheng, Jianhua Xu, Jin Q. Cheng, Jian-Yin Lin, Xu Ma

Published in: Child's Nervous System | Issue 1/2009

Abstract

Introduction

MicroRNAs (miRNAs) are small (∽22 nucleotides) regulatory RNAs which play fundamental roles in many human diseases, including cancer. There is no report on the miRNA expression profile of retinoblastoma.

Methods

This work was undertaken to identify differentially expressed miRNAs in human retinoblastoma tissues by microRNA microarray technique, and some miRNAs were verified using northern blot analysis and the in situ hybridization method.

Results

A cluster of microRNAs was identified as highly expressed in retinoblastoma, including hsa-miR-494, hsa-let-7e, hsa-miR-513–1, hsa-miR-513–2, hsa-miR-518c*, hsa-miR-129–1, hsa-miR-129–2, hsa-miR-198, hsa-miR-492, hsa-miR-498, hsa-miR-320, hsa-miR-503, and hsa-miR-373*.

Conclusion

These miRNAs are the first to be reported for human retinoblastoma and may play significant roles in regulating tumor genesis.

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Title: Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis
Authors: Jian-Jun Zhao
Juhua Yang
Jianhong Lin
Nan Yao
Yihua Zhu
Jianlong Zheng
Jianhua Xu
Jin Q. Cheng
Jian-Yin Lin
Xu Ma
Publication date: 01-01-2009
Publisher: Springer-Verlag
Published in: Child's Nervous System / Issue 1/2009
Print ISSN: 0256-7040
Electronic ISSN: 1433-0350
DOI: https://doi.org/10.1007/s00381-008-0701-x

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis

Abstract

Introduction

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Introduction

Methods

Results

Conclusion

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