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01-07-2014 | Original Article

Porphyromonas gingivalis promotes neointimal formation after arterial injury through toll-like receptor 2 signaling

Authors: Naho Kobayashi, Jun-ichi Suzuki, Masahito Ogawa, Norio Aoyama, Issei Komuro, Yuichi Izumi, Mitsuaki Isobe

Published in: Heart and Vessels | Issue 4/2014

Abstract

We previously demonstrated that Porphyromonas gingivalis infection induces neointimal hyperplasia with an increase in monocyte chemoattractant protein (MCP)-1 after arterial injury in wild-type mice. Toll-like receptor (TLR) 2 is a key receptor for the virulence factors of P. gingivalis. The aim of this study was to assess whether TLR2 plays a role in periodontopathic bacteria-induced neointimal formation after an arterial injury. Wild-type and TLR2-deficient mice were used in this study. The femoral arteries were injured, and P. gingivalis or vehicle was injected subcutaneously once per week. Fourteen days after arterial injury, the murine femoral arteries were obtained for histopathologic and immunohistochemical analyses. The immunoglobulin-G levels of the P. gingivalis-infected groups were significantly increased in comparison with the level in the corresponding noninfected groups in both wild-type and TLR2-deficient mice. TLR2 deficiency negated the P. gingivalis-induced neointimal formation in comparison with the wild-type mice, and reduced the number of positive monocyte chemoattractant protein-1 cells in the neointimal area. These findings demonstrate that P. gingivalis infection can promote neointimal formation after an arterial injury through TLR2 signaling.

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Title: Porphyromonas gingivalis promotes neointimal formation after arterial injury through toll-like receptor 2 signaling
Authors: Naho Kobayashi
Jun-ichi Suzuki
Masahito Ogawa
Norio Aoyama
Issei Komuro
Yuichi Izumi
Mitsuaki Isobe
Publication date: 01-07-2014
Publisher: Springer Japan
Published in: Heart and Vessels / Issue 4/2014
Print ISSN: 0910-8327
Electronic ISSN: 1615-2573
DOI: https://doi.org/10.1007/s00380-013-0405-3

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