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01-09-2012 | Original Article

Sulfur dioxide inhibits excessively activated endoplasmic reticulum stress in rats with myocardial injury

Authors: Shanshan Chen, Junbao Du, Yinfang Liang, Todd Ochs, Die Liu, Lulu Zhu, Xiuying Tang, Chaoshu Tang, Hongfang Jin

Published in: Heart and Vessels | Issue 5/2012

Abstract

It has been demonstrated that excessively activated endoplasmic reticulum stress (ERS) is related to myocardial injury. The study was designed to explore the possible role of sulfur dioxide (SO₂) in protecting excessively activated ERS in rats with isoproterenol (ISO)-induced myocardial injury. Wistar rats were randomly divided into control, ISO, and ISO + SO₂ groups. Cardiac catheterization-derived hemodynamic parameters and myocardial enzymes in plasma were measured. Microstructure changes in myocardial tissues were examined. Cardiomyocyte apoptosis was detected by TUNEL method. Myocardial SO₂ content and aspartate amino transferase (AAT) activity were detected. Meanwhile, protein and mRNA expressions of myocardial AAT1, AAT2, and ERS markers (GRP78, caspase-12, and CHOP) were evaluated. The results showed that cardiac function was decreased, myocardial microstructure was damaged, and myocardial enzyme levels and cardiomyocyte apoptosis were increased with a downregulated endogenous AAT/SO₂ pathway, and that ERS markers were upregulated at transcriptional and translational levels in ISO-treated rats. However, the administration of an SO₂ donor, resulting in an increased SO₂ content in myocardial tissues, improved cardiac function and myocardial structure, and ameliorated myocardial enzyme levels and cardiomyocyte apoptosis associated with a downregulation of excessively activated ERS. In conclusion, the endogenous AAT/SO₂ pathway was probably responsible for the inhibition of excessively activated ERS, which might be involved in the mechanism of ISO-induced myocardial injury.

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Title: Sulfur dioxide inhibits excessively activated endoplasmic reticulum stress in rats with myocardial injury
Authors: Shanshan Chen
Junbao Du
Yinfang Liang
Todd Ochs
Die Liu
Lulu Zhu
Xiuying Tang
Chaoshu Tang
Hongfang Jin
Publication date: 01-09-2012
Publisher: Springer Japan
Published in: Heart and Vessels / Issue 5/2012
Print ISSN: 0910-8327
Electronic ISSN: 1615-2573
DOI: https://doi.org/10.1007/s00380-011-0192-7

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