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European Radiology
Published in: European Radiology 9/2012

01-09-2012 | Molecular Imaging

In vivo MRI discrimination between live and lysed iron-labelled cells using balanced steady state free precession

Authors: E. J. Ribot, P. J. Foster

Published in: European Radiology | Issue 9/2012

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Abstract

Objectives

The goal of this study was to evaluate the ability of balanced steady state free precession (b-SSFP) magnetic resonance imaging sequence to distinguish between live and lysed iron-labelled cells.

Methods

Human breast cancer cells were labelled with iron oxide nanoparticles. Cells were lysed using sonication. Imaging was performed at 3 T. The timing parameters for b-SSFP and the number of iron-labelled cells in samples were varied to optimise the b-SSFP signal difference between live and lysed iron-labelled cell samples. For in vivo experiments, cells were mixed with Matrigel and implanted into nude mice. Three mice implanted with live labelled cancer cells were irradiated to validate this method.

Results

Lysed iron-labelled cells have a significantly higher signal compared with live, intact iron-labelled cells in bSSFP images. The contrast between live and dead cells can be maximised by careful optimisation of timing parameters. A change in the b-SSFP signal was measured 6 days after irradiation, reflecting cell death in vivo. Histology confirmed the presence of dead cells in the implant.

Conclusions

Our results show that the b-SSFP sequence can be optimised to allow for the discrimination of live iron-labelled cells and lysed iron-labelled cells in vitro and in vivo.

Key Points

• Balanced steady state free precession (b-SSFP) MRI can assess iron-labelled cells.
• bSSFP can discriminate between live and lysed iron-labelled cells.
• The compartmentalisation of iron in cells influences the bSSFP signal.
• The bSSFP signal increases after cell death by irradiation in vivo.
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Metadata
Title
In vivo MRI discrimination between live and lysed iron-labelled cells using balanced steady state free precession
Authors
E. J. Ribot
P. J. Foster
Publication date
01-09-2012
Publisher
Springer-Verlag
Published in
European Radiology / Issue 9/2012
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI
https://doi.org/10.1007/s00330-012-2435-0

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