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01-12-2010 | Experimental

Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography

Authors: M. A. Kuefner, S. Grudzenski, J. Hamann, S. Achenbach, Michael Lell, K. Anders, S. A. Schwab, L. Häberle, M. Löbrich, M. Uder

Published in: European Radiology | Issue 12/2010

Abstract

Aims

To compare in vivo DNA lesions induced during helical and sequential coronary computed tomography angiography (CTA) and to evaluate the effect of CT parameters on double-strand break (DSB) levels.

Methods

Thirty-six patients were examined with various CT protocols and modes (helical scan, n = 27; sequential scan, n = 9) either using a 64-slice dual-source or a 128-slice CT system. Blood samples were obtained before and 30 min after CT. Lymphocytes were isolated, stained against the phosphorylated histone variant γ-H2AX, and DSBs were visualised by using fluorescence microscopy.

Results

DSB yields 30 min after CTA ranged from 0.04 to 0.71 per cell and showed a significant correlation to DLP (ρ = 0.81, p < 0.00001). Median DSB yield and median DLP were significantly lower after sequential compared to helical CT examinations (0.11 vs. 0.37 DSBs/cell and 249 vs. 958 mGy cm, p < 0.00001). Additional calcium scoring led to an increase in DLP (p = 0.15) and DSB levels (p = 0.04). DSB levels normalised to the DLP showed a significant correlation to the attenuation of the blood (ρ = 0.53, p = 0.01) and a negative correlation to the body mass index of the patients (ρ = −0.37, p = 0.06).

Conclusion

γ-H2AX immunofluorescence microscopy allows one to determine dose-related effects on x-ray-induced DSB levels and to consider individual factors which cannot be monitored by physical dose measurements.

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Title: Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography
Authors: M. A. Kuefner
S. Grudzenski
J. Hamann
S. Achenbach
Michael Lell
K. Anders
S. A. Schwab
L. Häberle
M. Löbrich
M. Uder
Publication date: 01-12-2010
Publisher: Springer-Verlag
Published in: European Radiology / Issue 12/2010
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-010-1873-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography

Abstract

Aims

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

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Abstract

Aims

Methods

Results

Conclusion

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