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01-06-2018 | Observational Research

Association between memory B-cells and clinical and immunological features of primary Sjögren’s syndrome and Sicca patients

Authors: Filipe Barcelos, Catarina Martins, Ana Papoila, Carlos Geraldes, Joana Cardigos, Glória Nunes, Teresa Lopes, Nuno Alves, José Vaz-Patto, Jaime Branco, Luís-Miguel Borrego

Published in: Rheumatology International | Issue 6/2018

Abstract

B-cells play a pivotal role in primary Sjögren’s syndrome (pSS) pathogenesis. We aim to (1) evaluate the distribution of B-lymphocyte subpopulations in pSS and Sicca patients, (2) establish cut-off points that discriminate pSS from controls, (3) evaluate the association between memory B-cells and phenotypic features in pSS. We included 57 pSS patients, 68 Sicca and 24 healthy controls. Circulating B-cells were characterized by flow cytometry as naïve and memory subsets and classified from Bm1 to Bm5. Compared to controls, pSS patients had lower percentages (29.5 vs 44.4%) and absolute numbers (47 vs 106 cells/µl) of memory B-cells. Through ROC curves, a cut-off of ≤ 58 total memory B-cells/µl yielded a specificity of 0.88 and a sensitivity of 0.60 for pSS, and was met by 59.6% of pSS patients, 38.8% of Sicca and 12.5% of controls. A cut-off of < 23.5 Switched-memory B-cells/µl yielded a specificity of 0.88 and a sensitivity of 0.54 and was met by 54.4% of pSS patients, 37.3% of Sicca and 12.5% of controls. In pSS, lower total memory B-cells count was associated with longer disease duration (14.3 vs 8.1 years, p = 0.006) and more active disease profile, as evaluated by the European League Against Rheumatism (EULAR) Sjögren’s Syndrome Disease Activity Index (ESSDAI) (3.1 vs 1.4, p = 0.043). Decreased numbers of memory B-cells clearly discriminated pSS from controls and can also have prognostic value. It remains to be clarified whether Sicca patients with decreased memory B-cells represent pSS and if B-cell profiling could help in the diagnosis of pSS.

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Title: Association between memory B-cells and clinical and immunological features of primary Sjögren’s syndrome and Sicca patients
Authors: Filipe Barcelos
Catarina Martins
Ana Papoila
Carlos Geraldes
Joana Cardigos
Glória Nunes
Teresa Lopes
Nuno Alves
José Vaz-Patto
Jaime Branco
Luís-Miguel Borrego
Publication date: 01-06-2018
Publisher: Springer Berlin Heidelberg
Published in: Rheumatology International / Issue 6/2018
Print ISSN: 0172-8172
Electronic ISSN: 1437-160X
DOI: https://doi.org/10.1007/s00296-018-4018-0

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