Top

Published in:

01-07-2010 | Original Article

Effects of (−)-epigallocatechin-3-gallate on cyclooxygenase 2, PGE₂, and IL-8 expression induced by IL-1β in human synovial fibroblasts

Authors: Guo-Shu Huang, Ching-Ya Tseng, Chian-Her Lee, Sui-Long Su, Herng-Sheng Lee

Published in: Rheumatology International | Issue 9/2010

Abstract

The objective of this study was to examine the effects of (−)-epigallocatechin-3-gallate (EGCG) on cyclooxygenase 2 (COX-2), prostaglandin E₂ (PGE₂), and interleukin 8 (IL-8) expression induced by IL-1β in human synovial fibroblasts. Cells were enzymatically isolated from synovial tissue taken from patients undergoing joint replacement surgery for osteoarthritis. Reverse transcriptase-polymerase chain reaction, immunocytochemistry, and western blotting were used to assess the COX-2 gene and protein expression with the associated mechanisms. PGE₂ and IL-8 secretion into the culture medium was assayed by enzyme-linked immunosorbent assay. COX-2 upregulation in synovial fibroblasts induced by IL-1β was significantly suppressed by EGCG in a dose-dependent manner. PGE₂ and IL-8 secretion was also induced by IL-1β stimulation and significantly suppressed by EGCG. The mechanism was associated with the phosphorylation of IKKβ. EGCG may inhibit the expression of inflammatory mediators, such as COX-2, PGE₂, and IL-8, induced by IL-1β in human synovial fibroblasts. EGCG may be of value in the treatment of synovial inflammation.

Ahmed S, Pakozdi A, Koch AE (2006) Regulation of interleukin-1β-induced chemokine production and matrix metalloproteinase 2 activation by epigallocatechin-3-gallate in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum 54:2393–2401CrossRefPubMed

Muñoz-Fernández S, Martín J, Martín-Mola E, García-Rodriguez MC, Cantalejo M, Fontán G, Ferreira A (2001) Soluble HLA class I antigens in serum and synovial fluid from patients with rheumatoid arthritis and other arthropathies. Rheumatology 40:1365–1369CrossRefPubMed

Bröker BM, Edwards JC, Fanger MW, Lydyard PM (1999) The prevalence and distribution of macrophages bearing Fc gamma R I, Fc gamma R II, and Fc gamma R III in synovium. Scand J Rheumatol 19:123–135CrossRef

Eastgate JA, Symons JA, Wood NC, Grinlinton FM, di Giovine FS, Duff GW (1988) Correlation of plasma interleukin 1 levels with disease activity in rheumatoid arthritis. Lancet 2:706–709CrossRefPubMed

van Lent PL, Holthuysen AE, van den Bersselaar L, van Rooijen N, van de Putte LB, van den Berg WB (1995) Role of macrophage-like synovial lining cells in localization and expression of experimental arthritis. Scand J Rheumatol Suppl 101:83–89CrossRefPubMed

Kalliolias GD, Liossis SN (2008) The future of the IL-1 receptor antagonist anakinra: from rheumatoid arthritis to adult-onset Still’s disease and systemic-onset juvenile idiopathic arthritis. Expert Opin Invest Drugs 17:349–359CrossRef

Van der Kraan PM, van den Berg WB (2000) Anabolic and destructive mediators in osteoarthritis. Cur Opin Clin Nutr Metab Care 3:205–211CrossRef

Lee HS, Lee CH, Tsai HC, Salter DM (2009) Inhibition of cyclooxygenase 2 expression by diallyl sulfide on joint inflammation induced by urate crystal and IL-1beta. Osteoarthr Cartil. doi:10.1016/j.joca.2008.05.010

Chen D, Milacic V, Chen MS, Wan SB, Lam WH, Huo C, Landis-Piwowar KR, Cui QC, Wali A, Chan TH, Dou QP (2008) Tea polyphenols, their biological effects and potential molecular targets. Histol Histopathol 23:487–496PubMed

10.

Chen YF, Jobanputra P, Barton P, Bryan S, Fry-Smith A, Harris G, Taylor RS (2008) Cyclooxygenase-2 selective non-steroidal anti-inflammatory drugs (etodolac, meloxicam, celecoxib, rofecoxib, etoricoxib, valdecoxib and lumiracoxib) for osteoarthritis and rheumatoid arthritis: a systematic review and economic evaluation. Health Technol Assess 2:1–278

11.

Katiyar SK, Challa A, McCormick TS, Cooper KD, Mukhtar H (1999) Prevention of UVB-induced immunosuppression in mice by the green tea polyphenol (−)-epigallocatechin-3-gallate may be associated with alterations in IL-10 and IL-12 production. Carcinogenesis 20:2117–2124CrossRefPubMed

12.

Katiyar SK, Matsui MS, Elmets CA, Mukhtar H (1999) Polyphenolic antioxidant (−)-epigallocatechin-3-gallate from green tea reduces UVB-induced inflammatory responses and infiltration of leukocytes in human skin. Photochem Photobiol 69:148–153PubMed

13.

Katiyar SK, Afaq F, Perez A, Mukhtar H (2001) Green tea polyphenol (−)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress. Carcinogenesis 22:287–294CrossRefPubMed

14.

Katiyar SK, Mukhtar H (2001) Green tea polyphenol (−)-epigallocatechin-3-gallate treatment to mouse skin prevents UVB-induced infiltration of leukocytes, depletion of antigen-presenting cells, and oxidative stress. J Leukoc Biol 69:719–726PubMed

15.

Devika PT, Stanely Mainzen Prince P (2008) Protective effect of (−)-epigallocatechin-gallate (EGCG) on lipid peroxide metabolism in isoproterenol induced myocardial infarction in male Wistar rats: a histopathological study. Biomed Pharmacother 62:701–708CrossRefPubMed

16.

Ramadass P, Meerarani P, Toborek M, Robertson LW, Hennig B (2003) Dietary flavonoids modulate PCB-induced oxidative stress, CYP1A1 induction, and AhR-DNA binding activity in vascular endothelial cells. Toxicol Sci 76:212–219CrossRefPubMed

17.

Yang F, Oz HS, Barve S, de Villiers WJ, McClain CJ, Varilek GW (2001) The green tea polyphenol (−)-epigallocatechin-3-gallate blocks nuclear factor-kappa B activation by inhibiting I kappa B kinase activity in the intestinal epithelial cell line IEC-6. Mol Pharmacol 60:528–533PubMed

18.

Kim J, Hwang JS, Cho YK, Han Y, Jeon YJ, Yang KH (2001) Protective effects of (−)-epigallocatechin-3-gallate on UVA- and UVB-induced skin damage. Skin Pharmacol Appl Skin Physiol 14:11–19PubMed

19.

Albrecht DS, Clubbs EA, Ferruzzi M, Bomser JA (2008) Epigallocatechin-3-gallate (EGCG) inhibits PC-3 prostate cancer cell proliferation via MEK-independent ERK1/2 activation. Chem Biol Interact 171:89–95CrossRefPubMed

20.

Tang Y, Zhao DY, Elliott S, Zhao W, Curiel TJ, Beckman BS, Burow ME (2007) Epigallocatechin-3 gallate induces growth inhibition and apoptosis in human breast cancer cells through survivin suppression. Int J Oncol 31:705–711PubMed

21.

Fang MZ, Wang Y, Ai N, Hou Z, Sun Y, Lu H, Welsh W, Yang CS (2003) Tea polyphenol (−)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines. Cancer Res 63:7563–7570PubMed

22.

Nam S, Smith DM, Dou QP (2001) Ester bond-containing tea polyphenols potently inhibit proteasome activity in vitro and in vivo. J Biol Chem 276:13322–13330CrossRefPubMed

23.

Shimizu M, Deguchi A, Joe AK, Mckoy JF, Moriwaki H, Weinstein IB (2005) EGCG inhibits activation of HER3 and expression of cyclooxygenase-2 in human colon cancer cells. J Exp Ther Oncol 5:69–78PubMed

24.

Hussain T, Gupta S, Adhami VM, Mukhtar H (2005) Green tea constituent epigallocatechin-3-gallate selectively inhibits COX-2 without affecting COX-1 expression in human prostate carcinoma cells. Int J Cancer 113:660–669CrossRefPubMed

25.

Widlansky ME, Hamburg NM, Anter E, Holbrook M, Kahn DF, Elliott JG, Keaney JF Jr, Vita JA (2007) Acute EGCG supplementation reverses endothelial dysfunction in patients with coronary artery disease. J Am Coll Nutr 26:95–102PubMed

26.

Kris-Etherton PM, Lichtenstein AH, Howard BV, Steinberg D, Witztum JL (2004) Nutrition Committee of the American Heart Association Council on Nutrition, Physical Activity, and Metabolism. Antioxidant vitamin supplements and cardiovascular disease. Circulation 110:637–641CrossRefPubMed

27.

Lindblad S, Hedfors E (1987) Arthroscopic and immunohistologic characterization of knee joint synovitis in osteoarthritis. Arthritis Rheum 30:1081–1088CrossRefPubMed

28.

Ayral X, Pickering EH, Woodworth TG, Mackillop N, Dougados M (2005) Synovitis: a potential predictive factor of structural progression of medial tibiofemoral knee osteoarthritis—results of a 1 year longitudinal arthroscopic study in 422 patients. Osteoarthritis Cartilage 13:361–367CrossRefPubMed

29.

Smith MD, Triantafillou S, Parker A, Youssef PP, Coleman M (1997) Synovial membrane inflammation and cytokine production in patients with early osteoarthritis. J Rheumatol 24:365–371PubMed

30.

Pelletier JP, Martel-Pelletier J, Abramson SB (2001) Osteoarthritis, an inflammatory disease: potential implication for the selection of new therapeutic targets. Arthritis Rheum 44:1237–1247CrossRefPubMed

31.

Martel-Pelletier J, Pelletier JP, Fahmi H (2003) Cyclooxygenase-2 and prostaglandins in articular tissues. Semin Arthritis Rheum 33(3):155–167CrossRefPubMed

32.

Kaneko S, Satoh T, Chiba J, Ju C, Inoue K, Kagawa J (2000) Interleukin-6 and interleukin-8 levels in serum and synovial fluid of patients with osteoarthritis. Cytokines Cell Mol Ther 6:71–79CrossRefPubMed

33.

Ahmed S, Wang N, Lalonde M, Goldberg VM, Haqqi TM (2004) Green tea polyphenol epigallocatechin-3-gallate (EGCG) differentially inhibits interleukin-1 beta-induced expression of matrix metalloproteinase-1 and -13 in human chondrocytes. J Pharmacol Exp Ther 308:767–773CrossRefPubMed

34.

Adcocks C, Collin P, Buttle DJ (2002) Catechins from green tea (Camellia sinensis) inhibit bovine and human cartilage proteoglycan and type II collagen degradation in vitro. J Nutr 132:341–346PubMed

35.

Agro A, Langdon C, Smith F, Richards CD (1996) Prostaglandin E₂ enhances interleukin 8 (IL-8) and IL-6 but inhibits GMCSF production by IL-1 stimulated human synovial fibroblasts in vitro. J Rheumatol 23:862–868PubMed

Title: Effects of (−)-epigallocatechin-3-gallate on cyclooxygenase 2, PGE2, and IL-8 expression induced by IL-1β in human synovial fibroblasts
Authors: Guo-Shu Huang
Ching-Ya Tseng
Chian-Her Lee
Sui-Long Su
Herng-Sheng Lee
Publication date: 01-07-2010
Publisher: Springer-Verlag
Published in: Rheumatology International / Issue 9/2010
Print ISSN: 0172-8172
Electronic ISSN: 1437-160X
DOI: https://doi.org/10.1007/s00296-009-1128-8

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Effects of (−)-epigallocatechin-3-gallate on cyclooxygenase 2, PGE₂, and IL-8 expression induced by IL-1β in human synovial fibroblasts

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 9/2010

Oligo-secretory myeloma in a patient with ankylosing spondylitis

The Hebrew version of the FibroFatigue scale: validation of a questionnaire for assessment of fibromyalgia and chronic fatigue syndrome

Regulation of TNFα and IL1β in rheumatoid arthritis synovial fibroblasts by leukotriene B4

Nickel-induced allergy and contact dermatitis: does it induce autoimmunity and cutaneous sclerosis? An experimental study in Brown Norway rats

Clinical characteristics of Behçet’s disease in China

Polyarthritis with chondronecrosis associated with osteonecrosis, panniculitis and pancreatitis

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page