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01-10-2018 | Original Article

Red blood cells metabolome changes upon treatment with different X-ray irradiation doses

Authors: Fabio Baroni, Chiara Marraccini, Lucia Merolle, Vando Piccagli, Daniele Lambertini, Mauro Iori, Tommaso Fasano, Emanuela Casali, Alberto Spisni, Roberto Baricchi, Thelma A. Pertinhez

Published in: Annals of Hematology | Issue 10/2018

Abstract

The upholding of red blood cells (RBC) quality and the removal of leukocytes are two essential issues in transfusion therapy. Leukodepletion provides optimum results, nonetheless there are cases where irradiation is recommended for some groups of hematological patients such as the ones with chronic graft-vs-host disease, congenital cellular immunodeficiency, and hematopoietic stem cell transplant recipients. The European guidelines suggest irradiation doses from 25 to 50 Gray (Gγ). We evaluated the effect of different prescribed doses (15 to 50 Gγ) of X-ray irradiation on fresh leukodepleted RBCs bags using a novel protocol that provides a controlled irradiation. Biochemical assays integrated with RBCs metabolome profile, assessed by nuclear magnetic resonance spectroscopy, were performed on RBC units supernatant, during 14 days storage. Metabolome analysis evidenced a direct correlation between concentration increase of three metabolites, glycine, glutamine and creatine, and irradiation dose. Higher doses (35 and 50 Gγ) effect on RBC mean corpuscular volume, hemolysis, and ammonia concentration are considerable after 7 and 14 days of storage. Our data show that irradiation with 50 Gγ should be avoided and we suggest that 35 Gγ should be the upper limit. Moreover, we suggest for leukodepleted RBCs units the irradiation with the prescribed dose of 15 Gγ, value at center of bag, and ranging between 13.35–15 Gγ, measured over the entire bag volume, may guarantee the same benefits of a 25 Gγ dose assuring, in addition, a better quality of RBCs.

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Title: Red blood cells metabolome changes upon treatment with different X-ray irradiation doses
Authors: Fabio Baroni
Chiara Marraccini
Lucia Merolle
Vando Piccagli
Daniele Lambertini
Mauro Iori
Tommaso Fasano
Emanuela Casali
Alberto Spisni
Roberto Baricchi
Thelma A. Pertinhez
Publication date: 01-10-2018
Publisher: Springer Berlin Heidelberg
Published in: Annals of Hematology / Issue 10/2018
Print ISSN: 0939-5555
Electronic ISSN: 1432-0584
DOI: https://doi.org/10.1007/s00277-018-3386-6

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