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Annals of Hematology
Published in: Annals of Hematology 9/2016

01-09-2016 | Letter to the Editor

Droplet digital PCR assay for quantifying of CALR mutant allelic burden in myeloproliferative neoplasms

Authors: Luisa Anelli, Antonella Zagaria, Nicoletta Coccaro, Giuseppina Tota, Angela Minervini, Paola Casieri, Luciana Impera, Crescenzio Francesco Minervini, Claudia Brunetti, Alessandra Ricco, Paola Orsini, Cosimo Cumbo, Giorgina Specchia, Francesco Albano

Published in: Annals of Hematology | Issue 9/2016

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Excerpt

Dear Editor, …
Appendix
Available only for authorised users
Literature
1.
Nangalia J, Massie CE, Baxter EJ et al (2013) Somatic CALR mutations in myeloproliferative neoplasms with nonmutated JAK2. N Engl J Med 369:2391–2405CrossRefPubMedPubMedCentral
2.
Klampfl T, Gisslinger H, Harutyunyan AS et al (2013) Somatic mutations of calreticulin in myeloproliferative neoplasms. N Engl J Med 369:2379–2390CrossRefPubMed
3.
Jones AV, Ward D, Lyon M et al (2015) Evaluation of methods to detect CALR mutations in myeloproliferative neoplasms. Leuk Res 39:82–87CrossRefPubMed
4.
Chi J, Manoloukos M, Pierides C et al (2015) Calreticulin mutations in myeloproliferative neoplasms and new methodology for their detection and monitoring. Ann Hematol 94:399–408CrossRefPubMed
5.
Albano F, Zagaria A, Anelli L et al (2015) Absolute quantification of the pretreatment PML-RARA transcript defines the relapse risk in acute promyelocytic leukemia. Oncotarget 6:13269–13277CrossRefPubMedPubMedCentral
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Drandi D, Kubiczkova-Besse L, Ferrero S et al (2015) Minimal residual disease detection by droplet digital PCR in multiple myeloma, mantle cell lymphoma, and follicular lymphoma: a comparison with real-time PCR. J Mol Diagn 17:652–660CrossRefPubMed
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Zagaria A, Anelli L, Coccaro N et al (2015) BCR-ABL1 e6a2 transcript in chronic myeloid leukemia: biological features and molecular monitoring by droplet digital PCR. Virchows Arch 467:357–363CrossRefPubMed
8.
Mansier O, Migeon M, Saint-Lézer A et al (2016) Quantification of the mutant CALR allelic burden by digital PCR: application to minimal residual disease evaluation after bone marrow transplantation. J Mol Diagn 18:68–74CrossRefPubMed
9.
Badbaran A, Fehse B, Christopeit M et al (2016) Digital-PCR assay for screening and quantitative monitoring of calreticulin (CALR) type-2 positive patients with myelofibrosis following allogeneic stem cell transplantation. Bone Marrow Transplant 51(6):872–873CrossRefPubMed
10.
Cassinat B, Verger E, Kiladjian JJ (2014) Interferon alfa therapy in CALR-mutated essential thrombocythemia. N Engl J Med 371:188–189CrossRefPubMed
Metadata
Title
Droplet digital PCR assay for quantifying of CALR mutant allelic burden in myeloproliferative neoplasms
Authors
Luisa Anelli
Antonella Zagaria
Nicoletta Coccaro
Giuseppina Tota
Angela Minervini
Paola Casieri
Luciana Impera
Crescenzio Francesco Minervini
Claudia Brunetti
Alessandra Ricco
Paola Orsini
Cosimo Cumbo
Giorgina Specchia
Francesco Albano
Publication date
01-09-2016
Publisher
Springer Berlin Heidelberg
Published in
Annals of Hematology / Issue 9/2016
Print ISSN: 0939-5555
Electronic ISSN: 1432-0584
DOI
https://doi.org/10.1007/s00277-016-2739-2

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