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Aesthetic Plastic Surgery
Published in: Aesthetic Plastic Surgery 2/2019

01-04-2019 | Fat Grafting | Original Article

Effects of Stromal Vascular Fraction on Breast Cancer Growth and Fat Engraftment in NOD/SCID Mice

Authors: Joon Seok Lee, PilSeon Eo, Min Chul Kim, Jae Bong Kim, Hee Kyung Jin, Jae-Sung Bae, Jae-hwan Jeong, Ho Yong Park, Jung Dug Yang

Published in: Aesthetic Plastic Surgery | Issue 2/2019

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Abstract

Background

To overcome unpredictable fat graft resorption, cell-assisted lipotransfer using stromal vascular fraction (SVF) has been introduced. However, its effect on cancer growth stimulation and its oncological safety are debatable. We investigated the effect of SVF on adjacent breast cancer and transplanted fat in a mouse model.

Methods

A breast cancer xenograft model was constructed by injecting 2 × 106 MDA-MB-231-luc breast cancer cells into the right lower back of 40 NOD/SCID mice. Two weeks later, cancer size was sorted according to signal density using an in vivo optical imaging system, and 36 mice were included. Human fat was extracted from the abdomen, and SVFs were isolated using a component isolator. The mice were divided into four groups: A, controls; B, injected with 30 μl SVF; C, injected with 0.5 ml fat and 30 μl saline; group D, injected with 0.5 ml fat and 30 μl SVF. Magnetic resonance imaging and three-dimensional micro-computed tomography volumetric analysis were performed at 4 and 8 weeks.

Results

Tumor volume was 43.6, 42.3, 48.7, and 42.4 mm3 at the initial time point and 6780, 5940, 6080, and 5570 mm3 at 8 weeks in groups A, B, C, and D, respectively. Fat graft survival volume after 8 weeks was 49.32% and 62.03% in groups C and D, respectively. At 2-month follow-up after fat grafting in the xenograft model, SVF injection showed an increased fat survival rate and did not increase the adjacent tumor growth significantly.

Conclusion

Fat grafting with SVF yields satisfactory outcome in patients who undergo breast reconstruction surgery.

No Level Assigned

This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
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Metadata
Title
Effects of Stromal Vascular Fraction on Breast Cancer Growth and Fat Engraftment in NOD/SCID Mice
Authors
Joon Seok Lee
PilSeon Eo
Min Chul Kim
Jae Bong Kim
Hee Kyung Jin
Jae-Sung Bae
Jae-hwan Jeong
Ho Yong Park
Jung Dug Yang
Publication date
01-04-2019
Publisher
Springer US
Published in
Aesthetic Plastic Surgery / Issue 2/2019
Print ISSN: 0364-216X
Electronic ISSN: 1432-5241
DOI
https://doi.org/10.1007/s00266-018-01304-2

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