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01-08-2013 | Original Paper

Enhanced migration of human bone marrow stromal cells in modified collagen hydrogels

Authors: Daniel Guenther, Alexandra Oks, Max Ettinger, Emmanouil Liodakis, Maximilian Petri, Christian Krettek, Michael Jagodzinski, Carl Haasper

Published in: International Orthopaedics | Issue 8/2013

Abstract

Purpose

Collagen I hydrogels are widely used as scaffolds for regeneration of articular cartilage defects. We hypothesised that ingrowth might be improved by removing the superficial layer of a compressed hydrogel. The control group consisted of the original unmodified product.

Methods

The migration of human bone marrow stromal cells (hBMSCs) into the hydrogel was evaluated by confocal microscopy. We quantified the DNA concentration of the hydrogel for each group and time point and evaluated the chondrogenic differentiation of cells.

Results

After one week, the detectable amount of cells at the depth of 26–50 μm was significantly higher in the modified matrix (MM) than in the non-modified matrix (NM) (p = 0.011). The maximum depth of penetration was 75 μm (NM) and 200 μm (MM). After three weeks, the maximum depth of penetration was 175 μm (NM) and 200 μm (MM). Likewise, at a depth of 0–25 μm the amount of detectable cells was significantly higher in the MM group (p = 0.003). After 14 days, the concentration of DNA was significantly higher in the samples of the MM than in the control group (p = 0.000). Staining of histological sections and labelling with collagen II antibodies showed that a chondrogenic differentiation of cells in the scaffold can occur during in vitro cultivation.

Conclusions

Removing the superficial layer is essential to ensuring proper ingrowth of cells within the compressed hydrogel. Compressed hydrogels contribute better to cartilage regeneration after surface modification.

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Title: Enhanced migration of human bone marrow stromal cells in modified collagen hydrogels
Authors: Daniel Guenther
Alexandra Oks
Max Ettinger
Emmanouil Liodakis
Maximilian Petri
Christian Krettek
Michael Jagodzinski
Carl Haasper
Publication date: 01-08-2013
Publisher: Springer Berlin Heidelberg
Published in: International Orthopaedics / Issue 8/2013
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-013-1894-5

At a glance: The STEP trials

Springer Medicine

Enhanced migration of human bone marrow stromal cells in modified collagen hydrogels

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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