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International Orthopaedics

Published in:

01-06-2013 | Original Paper

Proteomic analysis of synovial fluid: insight into the pathogenesis of knee osteoarthritis

Authors: Weixiong Liao, Zhongli Li, Hongli Wang, Jie Wang, Yangmu Fu, Xiaowei Bai

Published in: International Orthopaedics | Issue 6/2013

Abstract

Purpose

We conducted a proteomic analysis of synovial fluid (SF) to identify differentially expressed proteins and analyse their correlation with osteoarthritis (OA) severity. Our primary purpose was to gain insight into the pathogenesis of OA.

Methods

SF samples were acquired from 12 knee OA patients and 12 non-OA controls (ten had a meniscus injury, two had a discoid meniscus and all exhibited intact articular cartilage) and sequentially subjected to two-dimensional electrophoresis (2-DE). The radiographic grading of knee OA was performed using the Kellgren-Lawrence criteria. Differentially expressed proteins were identified by matrix-assisted laser desorption/ionisation time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS). Proteins of interest identified from SF were detected using an enzyme-linked immunosorbent assay (ELISA).

Results

A total of 31 protein spots showed significant differences (p < 0.05) between the sample groups; 25 of the 31 spots (80.6 %) were identified as proteins of interest. Among them 20 corresponded to up-regulation and five to down-regulation in OA samples. HLA-DR was one of the proteins up-regulated, which was confirmed by ELISA.

Conclusions

These observations have implications in delineating the protein expression underlying the pathogenesis of OA and facilitate further elucidation of molecular mechanisms involved in disease progression. Substantial alterations of the protein profile in SF may be associated with OA severity.

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Title: Proteomic analysis of synovial fluid: insight into the pathogenesis of knee osteoarthritis
Authors: Weixiong Liao
Zhongli Li
Hongli Wang
Jie Wang
Yangmu Fu
Xiaowei Bai
Publication date: 01-06-2013
Publisher: Springer-Verlag
Published in: International Orthopaedics / Issue 6/2013
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-012-1768-2

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Proteomic analysis of synovial fluid: insight into the pathogenesis of knee osteoarthritis

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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