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Cancer Immunology, Immunotherapy
Published in: Cancer Immunology, Immunotherapy 10/2019

Open Access 01-10-2019 | Lymphoma | Original Article

Presence of T cells directed against CD20-derived peptides in healthy individuals and lymphoma patients

Authors: Benoit Milcent, Nathalie Josseaume, Quentin Riller, Ilenia Giglioli, Emilia Rabia, Claire Deligne, Jean-Baptiste Latouche, Mohamad Hamieh, Alexandre Couture, Olivier Toutirais, Yu-Chun Lone, Raphaël Jeger-Madiot, Stéphanie Graff-Dubois, Sandy Amorim, Pascale Loiseau, Antoine Toubert, Pauline Brice, Catherine Thieblemont, Jean-Luc Teillaud, Sophie Sibéril

Published in: Cancer Immunology, Immunotherapy | Issue 10/2019

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Abstract

Preclinical and clinical studies have suggested that cancer treatment with antitumor antibodies induces a specific adaptive T cell response. A central role in this process has been attributed to CD4+ T cells, but the relevant T cell epitopes, mostly derived from non-mutated self-antigens, are largely unknown. In this study, we have characterized human CD20-derived epitopes restricted by HLA-DR1, HLA-DR3, HLA-DR4, and HLA-DR7, and investigated whether T cell responses directed against CD20-derived peptides can be elicited in human HLA-DR-transgenic mice and human samples. Based on in vitro binding assays to recombinant human MHC II molecules and on in vivo immunization assays in H-2 KO/HLA-A2+-DR1+ transgenic mice, we have identified 21 MHC II-restricted long peptides derived from intracellular, membrane, or extracellular domains of the human non-mutated CD20 protein that trigger in vitro IFN-γ production by PBMCs and splenocytes from healthy individuals and by PBMCs from follicular lymphoma patients. These CD20-derived MHC II-restricted peptides could serve as a therapeutic tool for improving and/or monitoring anti-CD20 T cell activity in patients treated with rituximab or other anti-CD20 antibodies.
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Metadata
Title
Presence of T cells directed against CD20-derived peptides in healthy individuals and lymphoma patients
Authors
Benoit Milcent
Nathalie Josseaume
Quentin Riller
Ilenia Giglioli
Emilia Rabia
Claire Deligne
Jean-Baptiste Latouche
Mohamad Hamieh
Alexandre Couture
Olivier Toutirais
Yu-Chun Lone
Raphaël Jeger-Madiot
Stéphanie Graff-Dubois
Sandy Amorim
Pascale Loiseau
Antoine Toubert
Pauline Brice
Catherine Thieblemont
Jean-Luc Teillaud
Sophie Sibéril
Publication date
01-10-2019
Publisher
Springer Berlin Heidelberg
Keyword
Lymphoma
Published in
Cancer Immunology, Immunotherapy / Issue 10/2019
Print ISSN: 0340-7004
Electronic ISSN: 1432-0851
DOI
https://doi.org/10.1007/s00262-019-02389-7

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