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01-04-2016 | Original Paper

ApaL1 urokinase and Taq1 vitamin D receptor gene polymorphisms in first-stone formers, recurrent stone formers, and controls in a Caucasian population

Authors: Serdar Aykan, Murat Tuken, Sezgin Gunes, Yigit Akin, Murat Ozturk, Serkan Seyhan, Emrah Yuruk, Mustafa Zafer Temiz, Ali Faik Yılmaz, Daniel P. Nguyen

Published in: Urolithiasis | Issue 2/2016

Abstract

The purpose of this study was to determine differences in genotype distribution and allele frequency of urokinase and vitamin D receptor (VDR) single nucleotide polymorphisms (SNPs) between first-stone formers, recurrent stone formers, and controls in a Caucasian population. A total of 86 first-stone formers, 78 recurrent stone formers, and 167 controls were included. Urokinase and VDR SNPs were tested by gene amplification followed by ApaL1 and Taq1 endonuclease digestion, respectively. Baseline variables, genotype, and allele frequencies were compared between the three groups, using descriptive statistics. Adjusted odds ratios were calculated to estimate the risk for recurrent urolithiasis associated with genotypes. We found that differences in the distribution of ApaL1 SNP and Taq1 SNP genotypes were statistically different between recurrent stone formers and first-stone formers, and between recurrent stone formers and controls. Allele frequency analysis showed that the T allele for ApaL1 SNP and the C allele for Taq1 SNP were significantly associated with recurrent urolithiasis. For Taq1 SNP, logistic regression analysis showed that the C/C genotype was associated with a more than threefold higher risk for recurrent urolithiasis. We conclude that ApaL1 and Taq1 SNPs of the urokinase and VDR genes are associated with recurrent urolithiasis in a Caucasian population.

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Title: ApaL1 urokinase and Taq1 vitamin D receptor gene polymorphisms in first-stone formers, recurrent stone formers, and controls in a Caucasian population
Authors: Serdar Aykan
Murat Tuken
Sezgin Gunes
Yigit Akin
Murat Ozturk
Serkan Seyhan
Emrah Yuruk
Mustafa Zafer Temiz
Ali Faik Yılmaz
Daniel P. Nguyen
Publication date: 01-04-2016
Publisher: Springer Berlin Heidelberg
Published in: Urolithiasis / Issue 2/2016
Print ISSN: 2194-7228
Electronic ISSN: 2194-7236
DOI: https://doi.org/10.1007/s00240-015-0813-1

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

ApaL1 urokinase and Taq1 vitamin D receptor gene polymorphisms in first-stone formers, recurrent stone formers, and controls in a Caucasian population

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

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