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Urolithiasis
Published in: Urolithiasis 3/2004

01-06-2004 | Original Paper

Association of vitamin d receptor genotypes with calcium excretion in nephrolithiatic subjects in northern India

Authors: Vandana Relan, Madhu Khullar, S. K. Singh, S. K. Sharma

Published in: Urolithiasis | Issue 3/2004

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Abstract

Our objective was to investigate the association between the vitamin D receptor (VDR) allelic variants (Bsm I and Fok I) and nephrolithiasis in northern India. A total of 150 nephrolithiatic patients and 100 age and sex matched controls were enrolled for study. A 10 ml blood sample was obtained for biochemical analysis and DNA isolation. In addition, 24 h urine samples were obtained from each patient for the estimation of calcium and creatinine. PCR was performed for the Bsm I and Fok I VDR variants. The association between Bsm I and Fok I VDR polymorphism and nephrolithiasis was investigated after digestion with restriction enzymes (3 U). The product was analysed on 3% agarose gel for Bsm I and 15% polyacrylamide gel for Fok I allelic variants. We did not observe any significant differences in the prevalence of either the Bsm I or Fok I VDR genotypes between stone formers and controls. The B allele was found to be more prevalent in hypercalciuric patients compared to controls and nephrolithiatic subjects. The subjects with the bb genotype exhibited a higher calcium excretion than the BB genotype. Patients with the F allele were also found to excrete higher urinary calcium. VDR genotypes may be associated with increased calcium excretion in hypercalciuric nephrolithiatic subjects.
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Metadata
Title
Association of vitamin d receptor genotypes with calcium excretion in nephrolithiatic subjects in northern India
Authors
Vandana Relan
Madhu Khullar
S. K. Singh
S. K. Sharma
Publication date
01-06-2004
Publisher
Springer-Verlag
Published in
Urolithiasis / Issue 3/2004
Print ISSN: 2194-7228
Electronic ISSN: 2194-7236
DOI
https://doi.org/10.1007/s00240-004-0414-x

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