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01-05-2013 | Experimental Study

Intradermal injection of GFP-producing adipose stromal cells promotes survival of random-pattern skin flaps in rats

Authors: Vasileios Karathanasis, Spyros Petrakis, Konstantina Topouridou, Eleni Koliakou, George Koliakos, Efterpi Demiri

Published in: European Journal of Plastic Surgery | Issue 5/2013

Abstract

Background

Tissue necrosis is a common complication in operations that use skin flaps for reconstructive surgery. Here we demonstrate the beneficial effect of autologous genetically modified adipose-derived stromal cells (ASCs) in the survival of random-pattern skin flaps.

Methods

ASCs were isolated from the inguinal fat pad of Wistar rats and genetically modified in order to permanently produce green fluorescent protein (GFP) using the Sleeping Beauty transposon technology. Autologous GFP-producing cells were then injected intradermally into random-pattern skin flaps planned on the dorsal area of rats.

Results

Injection of ASCs resulted in significant improvement of skin flap survival. Histological analysis showed that the connective tissue was almost intact in skin flaps treated with ASCs in contrast to disorganized tissues from mock-treated skin flaps. GFP ASCs were detected in the endothelium of blood vessels co-expressing the endothelial marker von Willebrand factor, thus suggesting that they promote blood vessel regeneration.

Conclusions

These findings indicate that transplantation of autologous GFP ASCs improve survival of skin flaps. This methodology suggests that the use of genetically modified ASCs producing, e.g., angiogenic factors may facilitate survival and integration of flaps in plastic surgery.

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Title: Intradermal injection of GFP-producing adipose stromal cells promotes survival of random-pattern skin flaps in rats
Authors: Vasileios Karathanasis
Spyros Petrakis
Konstantina Topouridou
Eleni Koliakou
George Koliakos
Efterpi Demiri
Publication date: 01-05-2013
Publisher: Springer-Verlag
Published in: European Journal of Plastic Surgery / Issue 5/2013
Print ISSN: 0930-343X
Electronic ISSN: 1435-0130
DOI: https://doi.org/10.1007/s00238-013-0810-y

At a glance: The STEP trials

Springer Medicine

Intradermal injection of GFP-producing adipose stromal cells promotes survival of random-pattern skin flaps in rats

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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