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Open Access 01-07-2020 | Original Research

Characterization of Skeletal Muscle Endocrine Control in an In Vitro Model of Myogenesis

Authors: Cecilia Romagnoli, Roberto Zonefrati, Preeti Sharma, Marco Innocenti, Luisella Cianferotti, Maria Luisa Brandi

Published in: Calcified Tissue International | Issue 1/2020

Abstract

Skeletal muscle has remarkable regenerative abilities regulated by a highly orchestrated process involving the activation of cellular and molecular responses, which are dependent on satellite cells. These cells maintain the stem cell population and provide numerous myogenic cells that proliferate, differentiate, fuse and lead to new myofiber formation for a functional contractile tissue. We have isolated and characterized satellite cells obtained from human biopsies and established an in vitro model of myogenesis, evaluating muscle regeneration, monitoring the dynamic increases of the specific myogenic regulatory factors and the final formation of multinucleated myofibers. As the skeletal muscle is an endocrine tissue able of producing many substances that can act on distant organs, and it can be physiologically modulated by a variety of hormones, we embarked in a project of characterization of muscle cell endocrinology machinery. The expression of a large array of hormone receptors was quantified during the process of myogenesis. The results obtained showed a significant and generalized increase of all the tested hormone receptors along the process of differentiation of human cultured cells from myoblasts to myocytes. Interestingly, also the production of the myokine irisin increased in a parallel manner. These findings point to the human cultured myoblasts as an ideal model to characterize the skeletal muscle endocrine machinery and its hormonal regulation.

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Title: Characterization of Skeletal Muscle Endocrine Control in an In Vitro Model of Myogenesis
Authors: Cecilia Romagnoli
Roberto Zonefrati
Preeti Sharma
Marco Innocenti
Luisella Cianferotti
Maria Luisa Brandi
Publication date: 01-07-2020
Publisher: Springer US
Published in: Calcified Tissue International / Issue 1/2020
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI: https://doi.org/10.1007/s00223-020-00678-3

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