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Published in: Calcified Tissue International 5/2008

01-05-2008

Follicle-Stimulating Hormone Does Not Impact Male Bone Mass In Vivo or Human Male Osteoclasts In Vitro

Authors: Veronique Ritter, Barbara Thuering, Pierre Saint Mezard, Ngoc-Hong Luong-Nguyen, Yves Seltenmeyer, Uwe Junker, Brigitte Fournier, Mira Susa, Frederic Morvan

Published in: Calcified Tissue International | Issue 5/2008

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Abstract

Bone loss in the elderly is mainly caused by osteoclast-induced bone resorption thought to be causally linked to the decline in estrogen and testosterone levels in females and males. Recently, involvement of follicle stimulating-hormone (FSH) in this process has been suggested to explain in part the etiology of the disease in females, whereas its role in males has never been examined. In this study, the direct impact of FSH on bone mass of 16-week-old C57BL/6J male mice by either daily intermittent application of 6 or 60 μg/kg of FSH or continuous delivery via miniosmotic pump of a dose of 6 μg/kg over the course of a month was assessed. Femoral peripheral quantitative computed tomographic and microcomputed tomographic analyses at 0, 2, and 4 weeks of FSH-treated mice did not reveal any differences in cancellous and cortical bone compared to sham-treated mice. FSH functionality was verified by demonstrating cAMP induction and activation of a cAMP-response element–containing reporter cell line by FSH. Furthermore, osteoclastogenesis from human mononuclear cell precursors and from RAW 264.7 cells was not affected by FSH (3, 10, 30 ng/mL) compared to control. No direct effect of FSH on gene regulation was observed by Affymetrix Gene Array on RAW 264.7 cells. Lastly, no expression of FSH receptor (FSHR) mRNA or FSHR was observed by quantitative polymerase chain reaction and Western blot in either human male osteoclasts or RAW 264.7 cells. These data show that FSH does not appear to modulate male bone mass regulation in vivo and does not act directly on osteoclastogenesis in vitro.
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Metadata
Title
Follicle-Stimulating Hormone Does Not Impact Male Bone Mass In Vivo or Human Male Osteoclasts In Vitro
Authors
Veronique Ritter
Barbara Thuering
Pierre Saint Mezard
Ngoc-Hong Luong-Nguyen
Yves Seltenmeyer
Uwe Junker
Brigitte Fournier
Mira Susa
Frederic Morvan
Publication date
01-05-2008
Publisher
Springer-Verlag
Published in
Calcified Tissue International / Issue 5/2008
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI
https://doi.org/10.1007/s00223-008-9134-5

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