Skip to main content
Top
Published in: Calcified Tissue International 1/2005

01-07-2005

Importance of Phosphorylation for Osteopontin Regulation of Biomineralization

Authors: A. Gericke, C. Qin, L. Spevak, Y. Fujimoto, W. T. Butler, E. S. Sørensen, A. L. Boskey

Published in: Calcified Tissue International | Issue 1/2005

Login to get access

Abstract

Previous in vitro and in vivo studies demonstrated that osteopontin (OPN) is an inhibitor of the formation and growth of hydroxyapatite (HA) and other biominerals. The present study tests the hypotheses that the interaction of OPN with HA is determined by the extent of protein phosphorylation and that this interaction regulates the mineralization process. Bone OPN as previously reported inhibited HA formation and HA-seeded growth in a gelatin-gel system. A transglutaminase-linked OPN polymer had similar effects. Recombinant, nonphosphorylated OPN and chemically dephosphorylated OPN, had no effect on HA formation or growth in this system. In contrast, highly phosphorylated milk OPN (mOPN) promoted HA formation. The mOPN stabilized the conversion of amorphous calcium phosphate (a noncrystalline constituent of milk) to HA, whereas bone OPN had a lesser effect on this conversion. Mixtures of OPN and osteocalcin known to form a complex in vitro, unexpectedly promoted HA formation. To test the hypothesis that small alterations in protein conformation caused by phosphorylation account for the differences in the observed ability of OPN to interact with HA, the conformation of bone OPN and mOPN in the presence and absence of crystalline HA was determined by attenuated total reflection (ATR) infrared (IR) spectroscopy. Both proteins exhibited a predominantly random coil structure, which was unaffected by the addition of Ca2+. Binding to HA did not alter the secondary structure of bone OPN, but induced a small increase of β-sheet (few percent) in mOPN. These data taken together suggest that the phosphorylation of OPN is an important factor in regulating the OPN-mediated mineralization process.
Literature
1.
go back to reference Denhardt, D, Noda, M 1998Osteopontin expression and function: role in bone remodelingJ Cell Biochem30-3192102CrossRef Denhardt, D, Noda, M 1998Osteopontin expression and function: role in bone remodelingJ Cell Biochem30-3192102CrossRef
2.
go back to reference Giachelli, CM, Steitz, S 2000Osteopontin: a versatile regulator of inflammation and biomineralizationMatrix Biol19615622CrossRefPubMed Giachelli, CM, Steitz, S 2000Osteopontin: a versatile regulator of inflammation and biomineralizationMatrix Biol19615622CrossRefPubMed
3.
go back to reference Gokhale, J, Robey, PG, Boskey, AL 2001The Biochemistry of boneMarcus, RFeldman, DKelsey, J eds. Osteoporosis, Second Edition vol 1Academic PressSan Diego107189 Gokhale, J, Robey, PG, Boskey, AL 2001The Biochemistry of boneMarcus, RFeldman, DKelsey, J eds. Osteoporosis, Second Edition vol 1Academic PressSan Diego107189
4.
go back to reference Sodek, J, Ganss, B, McKee, MD 2000OsteopontinCrit Rev Oral Biol Med11279303PubMed Sodek, J, Ganss, B, McKee, MD 2000OsteopontinCrit Rev Oral Biol Med11279303PubMed
5.
go back to reference Fernandez, MS, Escobar, C, Lavelin, I, Pines, M, Arias, JL 2003Localization of osteopontin in oviduct tissue and eggshell during different stages of the avian egg laying cycleJ Struct Biol143171180CrossRefPubMed Fernandez, MS, Escobar, C, Lavelin, I, Pines, M, Arias, JL 2003Localization of osteopontin in oviduct tissue and eggshell during different stages of the avian egg laying cycleJ Struct Biol143171180CrossRefPubMed
6.
go back to reference Oldberg, A, Franzen, A, Heinegard, D 1986Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell-binding sequenceProc Natl Acad Sci USA8388198823PubMed Oldberg, A, Franzen, A, Heinegard, D 1986Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell-binding sequenceProc Natl Acad Sci USA8388198823PubMed
7.
go back to reference Prince, CW, Oosawa, T, Butler, WT, Tomana, M, Bhown, AS, Bhown, M, Schrohenloher, RE 1987Isolation, characterization, and biosynthesis of a phosphorylated glycoprotein from rat boneJ Biol Chem26229002907PubMed Prince, CW, Oosawa, T, Butler, WT, Tomana, M, Bhown, AS, Bhown, M, Schrohenloher, RE 1987Isolation, characterization, and biosynthesis of a phosphorylated glycoprotein from rat boneJ Biol Chem26229002907PubMed
8.
go back to reference Butler, WT 1995Structural and functional domains of osteopontinAnn N Y Acad Sci760611PubMed Butler, WT 1995Structural and functional domains of osteopontinAnn N Y Acad Sci760611PubMed
9.
go back to reference Chang, PL, Prince, CW 19941 Alpha, 25-dihydroxyvitamin D3 stimulates synthesis and secretion of nonphosphorylated osteopontin (secreted phosphoprotein 1) in mouse JB6 epidermal cellsCancer Res5121442150 Chang, PL, Prince, CW 19941 Alpha, 25-dihydroxyvitamin D3 stimulates synthesis and secretion of nonphosphorylated osteopontin (secreted phosphoprotein 1) in mouse JB6 epidermal cellsCancer Res5121442150
10.
go back to reference Noda, M, Vogel, RL, Craig, AM, Prahl, J, DeLuca, HF, Denhardt, DT 1990Identification of a DNA sequence responsible for binding of the 1,25-dihydroxyvitamin D3 receptor and 1,25-dihydroxyvitamin D3 enhancement of mouse secreted phosphoprotein 1 (SPP-1 or osteopontin) gene expressionProc Natl Acad Sci USA8799959999PubMed Noda, M, Vogel, RL, Craig, AM, Prahl, J, DeLuca, HF, Denhardt, DT 1990Identification of a DNA sequence responsible for binding of the 1,25-dihydroxyvitamin D3 receptor and 1,25-dihydroxyvitamin D3 enhancement of mouse secreted phosphoprotein 1 (SPP-1 or osteopontin) gene expressionProc Natl Acad Sci USA8799959999PubMed
11.
go back to reference Beck, GR,Jr, Zerler, B, Moran, E 2000Phosphate is a specific signal for induction of osteopontin gene expressionProc Natl Acad Sci USA9783528357CrossRefPubMed Beck, GR,Jr, Zerler, B, Moran, E 2000Phosphate is a specific signal for induction of osteopontin gene expressionProc Natl Acad Sci USA9783528357CrossRefPubMed
12.
go back to reference Noda, M, Rodan, GA 1989Transcriptional regulation of osteopontin production in rat osteoblast-like cells by parathyroid hormoneJ Biol Chem108713718 Noda, M, Rodan, GA 1989Transcriptional regulation of osteopontin production in rat osteoblast-like cells by parathyroid hormoneJ Biol Chem108713718
13.
go back to reference Prince, CW, Dickie, D, Krumdieck, CL 1991Osteopontin, a substrate for transglutaminase and factor XIII activityBiochem Biophys Res Commun17712051210CrossRefPubMed Prince, CW, Dickie, D, Krumdieck, CL 1991Osteopontin, a substrate for transglutaminase and factor XIII activityBiochem Biophys Res Commun17712051210CrossRefPubMed
14.
go back to reference Sørensen, ES, Rasmussen, LK, Møller, L, Jensen, PH, Hørup, P, Petersen, TE 1994Localization of transglutaminase-reactive glutamine residues in bovine osteopontinJ Biochem3041316 Sørensen, ES, Rasmussen, LK, Møller, L, Jensen, PH, Hørup, P, Petersen, TE 1994Localization of transglutaminase-reactive glutamine residues in bovine osteopontinJ Biochem3041316
15.
go back to reference Kaartinen, MT, Pirhonen, A, Linnala-Kankkunen, A, Mäenpää, PH 1997Transglutaminase-catalyzed cross-linking of osteopontin is inhibited by osteocalcinJ Biol Chem2722273622741CrossRefPubMed Kaartinen, MT, Pirhonen, A, Linnala-Kankkunen, A, Mäenpää, PH 1997Transglutaminase-catalyzed cross-linking of osteopontin is inhibited by osteocalcinJ Biol Chem2722273622741CrossRefPubMed
16.
go back to reference Kaartinen, MT, Pirhonen, A, Linnala-Kankkunen, A, Mäenpää, PH 1999Transglutaminase treated osteopontin exhibits increased collagen binding propertiesBiol Chem27417291735CrossRef Kaartinen, MT, Pirhonen, A, Linnala-Kankkunen, A, Mäenpää, PH 1999Transglutaminase treated osteopontin exhibits increased collagen binding propertiesBiol Chem27417291735CrossRef
17.
go back to reference Kaartinen, MT, El-Maadawy, S, Räsänen, NH, McKee, MD 2002Transglutaminase and its substrates in boneJ Bone Miner Res1221612173 Kaartinen, MT, El-Maadawy, S, Räsänen, NH, McKee, MD 2002Transglutaminase and its substrates in boneJ Bone Miner Res1221612173
18.
go back to reference Ikeda, S, Nomura, A, Yamaguchi, T, Suda, T, Yoshiki, S 1992In situ hybridization of bone matrix proteins in undecalcified adult rat bone sectionsJ Histochem Cytochem4010791088PubMed Ikeda, S, Nomura, A, Yamaguchi, T, Suda, T, Yoshiki, S 1992In situ hybridization of bone matrix proteins in undecalcified adult rat bone sectionsJ Histochem Cytochem4010791088PubMed
19.
go back to reference Prince, CW, Butler, WT 19871,25-Dihydroxyvitamin D3 regulates the biosynthesis of osteopontin, a bone-derived cell attachment protein, in clonal osteoblast-like osteosarcoma cellsCollagen Rel Res7305313 Prince, CW, Butler, WT 19871,25-Dihydroxyvitamin D3 regulates the biosynthesis of osteopontin, a bone-derived cell attachment protein, in clonal osteoblast-like osteosarcoma cellsCollagen Rel Res7305313
20.
go back to reference Yamate, T, Mocharla, H, Taguchi, Y, Igietseme, JU, Manoiagas, SC, Abe, E 1997Osteopontin expression by osteoclast and osteoblast progenitors in the murine bone marrow: demonstration of its requirement for osteoclastogenesis and its increase after ovariectomyEndocrinology13830473055CrossRefPubMed Yamate, T, Mocharla, H, Taguchi, Y, Igietseme, JU, Manoiagas, SC, Abe, E 1997Osteopontin expression by osteoclast and osteoblast progenitors in the murine bone marrow: demonstration of its requirement for osteoclastogenesis and its increase after ovariectomyEndocrinology13830473055CrossRefPubMed
21.
go back to reference Ishijima, M, Rittling, SR, Yamashita, T, Tsuji, K, Kurosawa, H, Nifuji, A, Denhardt, DT, Noda, M 2001Enhancement of osteoclastic bone resorption and suppression of osteoblastic bone formation in response to reduced mechanical stress do not occur in the absence of osteopontinJ Exp Med193399404CrossRefPubMed Ishijima, M, Rittling, SR, Yamashita, T, Tsuji, K, Kurosawa, H, Nifuji, A, Denhardt, DT, Noda, M 2001Enhancement of osteoclastic bone resorption and suppression of osteoblastic bone formation in response to reduced mechanical stress do not occur in the absence of osteopontinJ Exp Med193399404CrossRefPubMed
22.
go back to reference Ihara, H, Denhardt, DT, Furuya, K, Yamashita, T, Muguruma, Y, Tsuji, K, Hruska, KA, Higashio, K, Enomoto, S, Nifuji, A, Rittling, SR, Noda, M 2001Parathyroid hormone-induced bone resorption does not occur in the absence of osteopontinJ Biol Chem2761306513071CrossRefPubMed Ihara, H, Denhardt, DT, Furuya, K, Yamashita, T, Muguruma, Y, Tsuji, K, Hruska, KA, Higashio, K, Enomoto, S, Nifuji, A, Rittling, SR, Noda, M 2001Parathyroid hormone-induced bone resorption does not occur in the absence of osteopontinJ Biol Chem2761306513071CrossRefPubMed
23.
go back to reference Rittling, SR, Matsumoto, HN, McKee, MD, Nanci, A, An, XR, Novick, KE, Kowalski, AJ, Noda, M, Denhardt, DT 1998Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitroJ Bone Miner Res1311011111PubMed Rittling, SR, Matsumoto, HN, McKee, MD, Nanci, A, An, XR, Novick, KE, Kowalski, AJ, Noda, M, Denhardt, DT 1998Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitroJ Bone Miner Res1311011111PubMed
24.
go back to reference Yoshitake, H, Rittling, SR, Denhardt, DT, Noda, M 1999Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorptionProc Natl Acad Sci USA9781568160CrossRef Yoshitake, H, Rittling, SR, Denhardt, DT, Noda, M 1999Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorptionProc Natl Acad Sci USA9781568160CrossRef
25.
go back to reference Boskey, AL, Spevak, L, Paschalis, E, Doty, SB, McKee, MD 2002Osteopontin deficiency increases mineral content and mineral crystallinity in mouse boneCalcif Tissue Int71145154CrossRefPubMed Boskey, AL, Spevak, L, Paschalis, E, Doty, SB, McKee, MD 2002Osteopontin deficiency increases mineral content and mineral crystallinity in mouse boneCalcif Tissue Int71145154CrossRefPubMed
26.
go back to reference Qiu, SR, Wierzbicki, A, Orme, CA, Cody, AM, Hoyer, JR, Nancollas, GH, Zepeda, S, Yoreo, JJ 2004Molecular modulation of calcium oxalate crystallization by osteopontin and citrateProc Natl Acad Sci USA10118111815CrossRefPubMed Qiu, SR, Wierzbicki, A, Orme, CA, Cody, AM, Hoyer, JR, Nancollas, GH, Zepeda, S, Yoreo, JJ 2004Molecular modulation of calcium oxalate crystallization by osteopontin and citrateProc Natl Acad Sci USA10118111815CrossRefPubMed
27.
go back to reference Hincke, MT, St. Maurice, M 2000Phosphorylation-dependent modulation of calcium carbonate precipitation by chicken eggshell matrix proteinsGoldberg, MBoskey, ARobinson, C eds. Chemistry and Biology of Mineralized Tissues, Am Acad Orthop SurgRosemontIL1317 Hincke, MT, St. Maurice, M 2000Phosphorylation-dependent modulation of calcium carbonate precipitation by chicken eggshell matrix proteinsGoldberg, MBoskey, ARobinson, C eds. Chemistry and Biology of Mineralized Tissues, Am Acad Orthop SurgRosemontIL1317
28.
go back to reference Asou, Y, Rittling, SR, Yoshitake, H, Tsuji, K, Shinomiya, K, Nifuji, A, Denhardt, DT, Noda, M 2001Osteopontin facilitates angiogenesis, accumulation of osteoclasts, and resorption in ectopic boneEndocrinology14213251332CrossRefPubMed Asou, Y, Rittling, SR, Yoshitake, H, Tsuji, K, Shinomiya, K, Nifuji, A, Denhardt, DT, Noda, M 2001Osteopontin facilitates angiogenesis, accumulation of osteoclasts, and resorption in ectopic boneEndocrinology14213251332CrossRefPubMed
29.
go back to reference Wada, T, McKee, MD, Steitz, S, Giachelli, CM 1999Calcification of vascular smooth muscle cell cultures: inhibition by osteopontinCirc Res35166178 Wada, T, McKee, MD, Steitz, S, Giachelli, CM 1999Calcification of vascular smooth muscle cell cultures: inhibition by osteopontinCirc Res35166178
30.
go back to reference Neame, PJ, Butler, WT 1996Posttranslational modification in rat bone osteopontinConnect Tissue Res35145150PubMed Neame, PJ, Butler, WT 1996Posttranslational modification in rat bone osteopontinConnect Tissue Res35145150PubMed
31.
go back to reference Salih, E, Wang, J, Mah, J, Fluckiger, R 2002Natural variation in the extent of phosphorylation of bone phosphoproteins as a function of in vivo new bone formation induced by demineralized bone matrix in soft tissue and bony environmentsBiochem J364465474CrossRefPubMed Salih, E, Wang, J, Mah, J, Fluckiger, R 2002Natural variation in the extent of phosphorylation of bone phosphoproteins as a function of in vivo new bone formation induced by demineralized bone matrix in soft tissue and bony environmentsBiochem J364465474CrossRefPubMed
32.
go back to reference Qin, C, Baba, O, Butler, WT 2004Post-translational modifications of sibling proteins and their roles in osteogenesis and dentinogenesisCrit Rev Oral Biol Med15126136PubMed Qin, C, Baba, O, Butler, WT 2004Post-translational modifications of sibling proteins and their roles in osteogenesis and dentinogenesisCrit Rev Oral Biol Med15126136PubMed
33.
go back to reference Safran, JB, Butler, WT, Farach-Carson, MC 1998Modulation of osteopontin post-translational state by 1, 25-(OH)2-vitamin D3 Dependence on Ca2+ influxJ Biol Chem2732993529941CrossRefPubMed Safran, JB, Butler, WT, Farach-Carson, MC 1998Modulation of osteopontin post-translational state by 1, 25-(OH)2-vitamin D3 Dependence on Ca2+ influxJ Biol Chem2732993529941CrossRefPubMed
34.
go back to reference Fujusawa, Y, Kuboki, S, Sasaki, S 1986Changes in interaction of bovine dentin phosphophory with calcium and hydroxyapatite by chemical modificationCalcif Tissue Int39248251PubMed Fujusawa, Y, Kuboki, S, Sasaki, S 1986Changes in interaction of bovine dentin phosphophory with calcium and hydroxyapatite by chemical modificationCalcif Tissue Int39248251PubMed
35.
go back to reference Boskey, AL, Maresca, M, Ullrich, W, Doty, SB, Butler, WT, Prince, CW 1993Osteopontin-hydroxyapatite interactions in vitro: inhibition of hydroxyapatite formation and growth in a gelatin-gelBone Miner22147159PubMed Boskey, AL, Maresca, M, Ullrich, W, Doty, SB, Butler, WT, Prince, CW 1993Osteopontin-hydroxyapatite interactions in vitro: inhibition of hydroxyapatite formation and growth in a gelatin-gelBone Miner22147159PubMed
36.
go back to reference Hunter, GK, Kyle, CL, Goldberg, HA 1994Modulation of crystal formation by bone phosphoproteins: structural specificity of the osteopontin-mediated inhibition of hydroxyapatite formationBiochem J300723728PubMed Hunter, GK, Kyle, CL, Goldberg, HA 1994Modulation of crystal formation by bone phosphoproteins: structural specificity of the osteopontin-mediated inhibition of hydroxyapatite formationBiochem J300723728PubMed
37.
go back to reference Endo, A, Glimcher, MJ 1989The effect of complexing phosphoproteins to decalcified collagen on in vitro calcificationConnect Tissue Res21179190PubMed Endo, A, Glimcher, MJ 1989The effect of complexing phosphoproteins to decalcified collagen on in vitro calcificationConnect Tissue Res21179190PubMed
38.
go back to reference Boskey, AL, Doty, SB, Binderman, I 1994Adenosine 5-triphosphate promotes mineralization in differentiating chick limb-bud mesenchymal cell culturesMicrosc Res Tech28492504CrossRefPubMed Boskey, AL, Doty, SB, Binderman, I 1994Adenosine 5-triphosphate promotes mineralization in differentiating chick limb-bud mesenchymal cell culturesMicrosc Res Tech28492504CrossRefPubMed
39.
go back to reference Weber, GF, Zawaideh, S, Hikita, S, Kumar, VA, Cantor, H, Ashkar, S 2002Phosphorylation-dependent interaction of osteopontin with its receptors regulates macrophage migration and activationJ Leukoc Biol72752764PubMed Weber, GF, Zawaideh, S, Hikita, S, Kumar, VA, Cantor, H, Ashkar, S 2002Phosphorylation-dependent interaction of osteopontin with its receptors regulates macrophage migration and activationJ Leukoc Biol72752764PubMed
40.
go back to reference Razzouk, S, Brunn, JC, Qin, C, Tye, CE, Goldberg, HA, Butler, WT 2002Osteopontin posttranslational modifications, possibly phosphorylation, are required for in vitro bone resorption but not osteoclast adhesionBone304047CrossRefPubMed Razzouk, S, Brunn, JC, Qin, C, Tye, CE, Goldberg, HA, Butler, WT 2002Osteopontin posttranslational modifications, possibly phosphorylation, are required for in vitro bone resorption but not osteoclast adhesionBone304047CrossRefPubMed
41.
go back to reference Addadi, L, Weiner, S, Geva, M 2001On how proteins, interact with crystals and their effect on crystal formationZ Kardiol 90 Suppl39298 Addadi, L, Weiner, S, Geva, M 2001On how proteins, interact with crystals and their effect on crystal formationZ Kardiol 90 Suppl39298
42.
go back to reference Winkler, S, Wilson, D, Kaplan, DL 2000Controlling beta-sheet assembly in genetically engineereds silk by enzymatic phosphorylation/dephosphorylationBiochemistry391273912746CrossRefPubMed Winkler, S, Wilson, D, Kaplan, DL 2000Controlling beta-sheet assembly in genetically engineereds silk by enzymatic phosphorylation/dephosphorylationBiochemistry391273912746CrossRefPubMed
43.
go back to reference Qin, C, Brunn, JC, Jones, J, George, A, Ramachandran, A, Gorski, JP, Butler, WT 2001A comparative study of sialic acid-rich proteins in rat bone and dentinEur J Oral Sci109133141CrossRefPubMed Qin, C, Brunn, JC, Jones, J, George, A, Ramachandran, A, Gorski, JP, Butler, WT 2001A comparative study of sialic acid-rich proteins in rat bone and dentinEur J Oral Sci109133141CrossRefPubMed
44.
go back to reference Sorensen, ES, Hojrup, P, Petersen, TE 1995Posttranslational modifications of bovine osteopontin: identification of twenty-eight phosphorylation and three O-glycosylation sitesProteinSci420402049 Sorensen, ES, Hojrup, P, Petersen, TE 1995Posttranslational modifications of bovine osteopontin: identification of twenty-eight phosphorylation and three O-glycosylation sitesProteinSci420402049
45.
go back to reference Jonsson, K, McDevitt, D, McGavin, MH, Patti, JM, Hook, M 1995Staphylococcus aureus expresses a major histocompatibility complex class II analogJ Biol Chem2702145721460CrossRefPubMed Jonsson, K, McDevitt, D, McGavin, MH, Patti, JM, Hook, M 1995Staphylococcus aureus expresses a major histocompatibility complex class II analogJ Biol Chem2702145721460CrossRefPubMed
46.
go back to reference Baykov, AA, Evtushenko, OA, Avaeva, SM 1988A malachite green procedure for orthophosphate determination and, its use in alkaline phosphatase-based enzyme immunoassayAnal Biochem171266270CrossRefPubMed Baykov, AA, Evtushenko, OA, Avaeva, SM 1988A malachite green procedure for orthophosphate determination and, its use in alkaline phosphatase-based enzyme immunoassayAnal Biochem171266270CrossRefPubMed
47.
go back to reference Gundberg, CM, Hauschka, PV, Lian, JB, Gallop, PM 1984Osteocalcin: isolation, characterization, and detectionMethods Enzymol107516544PubMed Gundberg, CM, Hauschka, PV, Lian, JB, Gallop, PM 1984Osteocalcin: isolation, characterization, and detectionMethods Enzymol107516544PubMed
48.
go back to reference Boskey, AL 1989Hydroxyapatite formation in a dynamic gelatin gel systemJ Phys Chem9416281633CrossRef Boskey, AL 1989Hydroxyapatite formation in a dynamic gelatin gel systemJ Phys Chem9416281633CrossRef
49.
go back to reference Silverman, L, Boskey, AL 2004Diffusion systems for evaluation of biomineralizationCalcif Tissue Int75494501CrossRefPubMed Silverman, L, Boskey, AL 2004Diffusion systems for evaluation of biomineralizationCalcif Tissue Int75494501CrossRefPubMed
50.
go back to reference Blumenthal, NC, Betts, F, Posner, AS 1975Effect of carbonate and biological macromolecules on formation and properties of hydroxyapatiteCalcif Tissue Res188190PubMed Blumenthal, NC, Betts, F, Posner, AS 1975Effect of carbonate and biological macromolecules on formation and properties of hydroxyapatiteCalcif Tissue Res188190PubMed
51.
go back to reference Willis, JB 1960Determination of metals in blood serum by atomic absorption spectroscopyI. Calcium. Spectrocbim Acta16259272 Willis, JB 1960Determination of metals in blood serum by atomic absorption spectroscopyI. Calcium. Spectrocbim Acta16259272
52.
go back to reference Heinonen, JK, Lahti, RJ 1981A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatesAnal Biochem113313317CrossRefPubMed Heinonen, JK, Lahti, RJ 1981A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatesAnal Biochem113313317CrossRefPubMed
53.
go back to reference Boskey, AL, Posner, AS 1973Conversion of amorphous calcium phosphate to microcrystalline hydroxyapatite. A pH-dependent solution-mediated, solid-state conversionJ Phys Chem7723132317CrossRef Boskey, AL, Posner, AS 1973Conversion of amorphous calcium phosphate to microcrystalline hydroxyapatite. A pH-dependent solution-mediated, solid-state conversionJ Phys Chem7723132317CrossRef
54.
go back to reference Diem, M 1993Introduction to modern vibrational spectroscopyWileyNew York Diem, M 1993Introduction to modern vibrational spectroscopyWileyNew York
55.
go back to reference Boskey, AL 1995Osteopontin and related phosphorylated sialoproteins: effects on mineralizationAnn NY Acad Sci760249256PubMed Boskey, AL 1995Osteopontin and related phosphorylated sialoproteins: effects on mineralizationAnn NY Acad Sci760249256PubMed
56.
go back to reference Goldberg, HA, Warner, KJ, Li, MC, Hunter, GK 2001Binding of bone sialoprotein, osteopontin and synthetic polypeptides to hydroxyapatiteConnect Tissue Res422537PubMed Goldberg, HA, Warner, KJ, Li, MC, Hunter, GK 2001Binding of bone sialoprotein, osteopontin and synthetic polypeptides to hydroxyapatiteConnect Tissue Res422537PubMed
57.
go back to reference McGann, TC, Kearney, RD, Buchheim, W, Posner, AS, Betts, F, Blumenthal, NC 1983Amorphous calcium phosphate in casein micelles of bovine milkCalcif Tissue Int35821823PubMed McGann, TC, Kearney, RD, Buchheim, W, Posner, AS, Betts, F, Blumenthal, NC 1983Amorphous calcium phosphate in casein micelles of bovine milkCalcif Tissue Int35821823PubMed
58.
go back to reference Ritter, NM, Farach-Carson, MC, Butler, WT 1992Evidence for the formation of a complex between osteopontin and osteocalcinJ Bone Miner Res7877885PubMed Ritter, NM, Farach-Carson, MC, Butler, WT 1992Evidence for the formation of a complex between osteopontin and osteocalcinJ Bone Miner Res7877885PubMed
59.
go back to reference Fisher, LW, Torchia, DA, Fohr, B, Young, MF, Fedarko, NS 2001Flexible structures of SIBLING proteins, bone sialoprotein, and osteopontinBiochem Biophys Res Commun280460465CrossRefPubMed Fisher, LW, Torchia, DA, Fohr, B, Young, MF, Fedarko, NS 2001Flexible structures of SIBLING proteins, bone sialoprotein, and osteopontinBiochem Biophys Res Commun280460465CrossRefPubMed
60.
go back to reference Arrondo, JLR, Goni, FM 1999Structure and dynamics of membrane proteins as studied by infrared spectroscopyProg Biophys Mol Biol72367405CrossRefPubMed Arrondo, JLR, Goni, FM 1999Structure and dynamics of membrane proteins as studied by infrared spectroscopyProg Biophys Mol Biol72367405CrossRefPubMed
61.
go back to reference Haris, PI, Chapman, D 1996Fourier transform infrared spectroscopic studies of biomembrane systemsMantsch, HHChapman, D eds. Infrared spectroscopy of biomoleculesWileyNew York239279 Haris, PI, Chapman, D 1996Fourier transform infrared spectroscopic studies of biomembrane systemsMantsch, HHChapman, D eds. Infrared spectroscopy of biomoleculesWileyNew York239279
62.
go back to reference Hayman, AR, Cox, TM 2003Tartrate-resistant acid phosphatase knockout miceJ Bone Miner Res1819051907PubMed Hayman, AR, Cox, TM 2003Tartrate-resistant acid phosphatase knockout miceJ Bone Miner Res1819051907PubMed
63.
go back to reference Pampena, DA, Robertson, KA, Litvinova, O, Lajoie, G, Goldberg, HA, Hunter, GK 2004Inhibition of hydroxyapatite formation by osteopontin phosphopeptidesBiochem J37810831087CrossRefPubMed Pampena, DA, Robertson, KA, Litvinova, O, Lajoie, G, Goldberg, HA, Hunter, GK 2004Inhibition of hydroxyapatite formation by osteopontin phosphopeptidesBiochem J37810831087CrossRefPubMed
64.
go back to reference Ito, S, Saito, T, Amano, K 2004In vitro apatite induction by osteopontin: interfacial energy for hydroxyapatite nucleation on osteopontinJ Biomed Mater Res69A1116CrossRef Ito, S, Saito, T, Amano, K 2004In vitro apatite induction by osteopontin: interfacial energy for hydroxyapatite nucleation on osteopontinJ Biomed Mater Res69A1116CrossRef
65.
go back to reference Steitz, SA, Speer, MY, McKee, MD, Liaw, L, Almedia, M, Yang, H, Giachelli, CM 2002Osteopontin inhibits mineral deposition and promotes regression of ectopic calcificationAm J Pathol16120352046PubMed Steitz, SA, Speer, MY, McKee, MD, Liaw, L, Almedia, M, Yang, H, Giachelli, CM 2002Osteopontin inhibits mineral deposition and promotes regression of ectopic calcificationAm J Pathol16120352046PubMed
66.
go back to reference Jono, S, Peinado, C, Giachelli, CM 2000Phosphorylation of osteopontin is required for inhibition of vascular smooth muscle cell calcificationJ Biol Chem2752019720203CrossRefPubMed Jono, S, Peinado, C, Giachelli, CM 2000Phosphorylation of osteopontin is required for inhibition of vascular smooth muscle cell calcificationJ Biol Chem2752019720203CrossRefPubMed
67.
go back to reference Gorski, JP, Kremer, E, Ruiz-Perez, J, Wise, GE, Artigues, A 1995Conformational analyses on soluble and surface bound osteopontinAnn N Y Acad Sci7601223PubMed Gorski, JP, Kremer, E, Ruiz-Perez, J, Wise, GE, Artigues, A 1995Conformational analyses on soluble and surface bound osteopontinAnn N Y Acad Sci7601223PubMed
68.
go back to reference Johnson, K, Goding, J, Etten, D, Sali, A, Hu, SI, Farley, D, Krug, H, Hessle, L, Millan, JL, Terkeltaub, R 2003Linked deficiencies in extracellular PP (i) and osteopontin mediate pathologic calcification associated with defective PC-1 and ANK expressionJ Bone Miner Res189941004PubMed Johnson, K, Goding, J, Etten, D, Sali, A, Hu, SI, Farley, D, Krug, H, Hessle, L, Millan, JL, Terkeltaub, R 2003Linked deficiencies in extracellular PP (i) and osteopontin mediate pathologic calcification associated with defective PC-1 and ANK expressionJ Bone Miner Res189941004PubMed
Metadata
Title
Importance of Phosphorylation for Osteopontin Regulation of Biomineralization
Authors
A. Gericke
C. Qin
L. Spevak
Y. Fujimoto
W. T. Butler
E. S. Sørensen
A. L. Boskey
Publication date
01-07-2005
Publisher
Springer-Verlag
Published in
Calcified Tissue International / Issue 1/2005
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI
https://doi.org/10.1007/s00223-004-1288-1

Other articles of this Issue 1/2005

Calcified Tissue International 1/2005 Go to the issue
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.