Published in:
01-06-2003 | Laboratory Investigations
Volumes From Which Calcium and Phosphorus X-Rays Arise in Electron Probe Emission Microanalysis of Bone: Monte Carlo Simulation
Authors:
P. G. T. Howell, A. Boyde
Published in:
Calcified Tissue International
|
Issue 6/2003
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Abstract
Monte Carlo simulations of trajectories for electrons with initial energy of 10 keV through 30 keV were used to map the 3D location of characteristic x-ray photon production for the elements C, P, and Ca until the electrons either escaped as backscattered electrons (BSE) or had insignificant energy. The x-ray production volumes for phosphorus slightly exceed those for calcium, but both greatly exceed the volume through which BSE travel prior to leaving the sample. The x-ray volumes are roughly hemispherical in shape, and the oblate spheroid from which BSE derive occupies only the upper third to half the volume of x-ray generation. Energy-dispersive x-ray emission microanalysis (EDX) may not be secure as a method for the quantitation of BSE images of bone in the scanning electron microscope (SEM). Ca:P elemental ratios from EDX analyses may also be imperfect.