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01-03-2017 | Original Article

Cytosolic proteome profiling of monocytes for male osteoporosis

Authors: W. Zhu, H. Shen, J-G. Zhang, L. Zhang, Y. Zeng, H-L. Huang, Y-C. Zhao, H. He, Y. Zhou, K-H. Wu, Q. Tian, L-J. Zhao, F-Y. Deng, H-W. Deng

Published in: Osteoporosis International | Issue 3/2017

Abstract

Summary

In male Caucasians with discordant hip bone mineral density (BMD), we applied the subcellular separation and proteome profiling to investigate the monocytic cytosol. Three BMD-associated proteins (ALDOA, MYH14, and Rap1B) were identified based on multiple omics evidence, and they may influence the pathogenic mechanisms of osteoporosis by regulating the activities of monocytes.

Introduction

Osteoporosis is a serious public health problem, leading to significant mortality not only in aging females but also in males. Peripheral blood monocytes (PBMs) play important roles in bone metabolism by acting as precursors of osteoclasts and producing cytokines important for osteoclast development. The first cytosolic sub-proteome profiling analysis was performed in male PBMs to identify differentially expressed proteins (DEPs) that are associated with BMDs and risk of osteoporosis.

Methods

Here, we conducted a comparative proteomics analysis in PBMs from Caucasian male subjects with discordant hip BMD (29 low BMD vs. 30 high BMD). To decrease the proteome complexity and expand the coverage range of the cellular proteome, we separated the PBM proteome into several subcellular compartments and focused on the cytosolic fractions, which are involved in a wide range of fundamental biochemical processes.

Results

Of the total of 3796 detected cytosolic proteins, we identified 16 significant (P < 0.05) and an additional 22 suggestive (P < 0.1) DEPs between samples with low vs. high hip BMDs. Some of the genes for DEPs, including ALDOA, MYH14, and Rap1B, showed an association with BMD in multiple omics studies (proteomic, transcriptomic, and genomic). Further bioinformatics analysis revealed the enrichment of DEPs in functional terms for monocyte proliferation, differentiation, and migration.

Conclusions

The combination strategy of subcellular separation and proteome profiling allows an in-depth and refined investigation into the composition and functions of cytosolic proteome, which may shed light on the monocyte-mediated pathogenic mechanisms of osteoporosis.

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Title: Cytosolic proteome profiling of monocytes for male osteoporosis
Authors: W. Zhu
H. Shen
J-G. Zhang
L. Zhang
Y. Zeng
H-L. Huang
Y-C. Zhao
H. He
Y. Zhou
K-H. Wu
Q. Tian
L-J. Zhao
F-Y. Deng
H-W. Deng
Publication date: 01-03-2017
Publisher: Springer London
Published in: Osteoporosis International / Issue 3/2017
Print ISSN: 0937-941X
Electronic ISSN: 1433-2965
DOI: https://doi.org/10.1007/s00198-016-3825-y

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Cytosolic proteome profiling of monocytes for male osteoporosis

Abstract

Summary

Introduction

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Summary

Introduction

Methods

Results

Conclusions

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