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International Urogynecology Journal
Published in: International Urogynecology Journal 3/2014

01-03-2014 | Original Article

Urethral striated muscle and extracellular matrix morphological characteristics among mildly diabetic pregnant rats: translational approach

Authors: Fernanda Piculo, Gabriela Marini, Angélica Mércia Pascon Barbosa, Débora Cristina Damasceno, Selma Maria Michelin Matheus, Sérgio Luis Felisbino, Firouz Daneshgari, Marilza Vieira Cunha Rudge

Published in: International Urogynecology Journal | Issue 3/2014

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Abstract

Introduction and hypothesis

Diabetes mellitus (DM) during pregnancy is associated with high levels of urinary incontinence (UI) and pelvic floor muscle dysfunction. Mild DM can lead to changes in urethral striated muscle and extracellular matrix (ECM) in pregnant rats considering both structures as an entire system responsible for urinary continence.

Methods

Ninety-two female Wistar rats were distributed in four experimental groups: virgin, pregnant, diabetic, and diabetic pregnant. In adult life, parental nondiabetic female rats were mated with nondiabetic male rats to obtain newborns. At the first day of birth, newborns received citrate buffer (nondiabetic group) or streptozotocin 100 mg/kg body weight, subcutaneous route (mild DM group). At day 21 of the pregnancy, the rats were lethally anesthetized and the urethra and vagina were extracted as a unit. Urethral and vaginal sections were cut and analyzed by: (a) cytochemical staining for ECM and muscle structural components, (b) immunohistochemistry to identify fast- and slow-muscle fibers, and (c) transmission electron microscopy for ultrastructural analysis of urethral striated muscle.

Results

In comparison with the three control groups, variations in the urethral striated muscle and ECM from diabetic pregnant rats were observed including thinning, atrophy, fibrosis, increased area of blood vessels, mitochondria accumulation, increased lipid droplets, glycogen granules associated with colocalization of fast and slow fibers, and a steady decrease in the proportion of fast to slow fibers.

Conclusions

Mild DM and pregnancy can lead to a time-dependent disorder and tissue remodeling in which the urethral striated muscle and ECM has a fundamental function.
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Metadata
Title
Urethral striated muscle and extracellular matrix morphological characteristics among mildly diabetic pregnant rats: translational approach
Authors
Fernanda Piculo
Gabriela Marini
Angélica Mércia Pascon Barbosa
Débora Cristina Damasceno
Selma Maria Michelin Matheus
Sérgio Luis Felisbino
Firouz Daneshgari
Marilza Vieira Cunha Rudge
Publication date
01-03-2014
Publisher
Springer London
Published in
International Urogynecology Journal / Issue 3/2014
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI
https://doi.org/10.1007/s00192-013-2218-4

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