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01-02-2010 | Original Article

Metabolic profile of glycosaminoglycans in bladder and urethra of female rats during and after pregnancy

Authors: Presciliana Mitrano, Marie Doki Nogueira, Paulo Cezar Feldner Jr., Rodrigo Aquino Castro, Marair Gracio Ferreira Sartori, Helena B. Nader, Manoel João Batista Castello Girão

Published in: International Urogynecology Journal | Issue 2/2010

Abstract

Introduction and hypothesis

The objective of this study is to evaluate pregnancy’s action on glycosaminoglycans (GAGs) profile in bladder and urethra of female adult rats.

Methods

Twenty adult female rats were divided into four groups: control, day 10 pregnancy, day 20 pregnancy, and day 5 after delivery. Sulfated GAGs content were determined by densitometry. Hyaluronic acid was assessed by fluorimetric enzyme-linked immunosorbent serologic-like assay. Radioactive GAGs were quantified by measuring radioactivity in electrophoresis gel. Statistical analysis was performed using Kruskal–Wallis and Mann–Whitney tests.

Results

In all groups, dermatan sulfate was predominant followed by heparan sulfate and hyaluronic acid. Significant decrease in total GAGs in bladder was observed at the beginning of pregnancy, with progressive increase. Biosynthesis of such molecules showed a similar behavior. In urethra, changes in GAGs content were restricted to the end of pregnancy. Hyaluronic acid content showed a significant increase in bladder during puerperium.

Conclusions

We observed different composition in vesical and urethral tissues during pregnancy and in puerperium.

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Title: Metabolic profile of glycosaminoglycans in bladder and urethra of female rats during and after pregnancy
Authors: Presciliana Mitrano
Marie Doki Nogueira
Paulo Cezar Feldner Jr.
Rodrigo Aquino Castro
Marair Gracio Ferreira Sartori
Helena B. Nader
Manoel João Batista Castello Girão
Publication date: 01-02-2010
Publisher: Springer-Verlag
Published in: International Urogynecology Journal / Issue 2/2010
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-009-1001-z

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Metabolic profile of glycosaminoglycans in bladder and urethra of female rats during and after pregnancy

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

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