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International Urogynecology Journal

Published in:

01-06-2010 | Original Article

Autologous transplantation of adult adipose derived stem cells into rabbit urethral wall

Authors: Fernando Gonçalves Almeida, Yuri Tulio Dantas Nobre, Katia R. Leite, Homero Bruschini

Published in: International Urogynecology Journal | Issue 6/2010

Abstract

Introduction and hypothesis

A study was carried out to evaluate the feasibility of autologous adipose derived stem cells (ADSC) transplantation into female rabbits' urethra walls as an alternative to intrinsic urethral regeneration.

Methods

Inguinal fat pad of 12 New Zealand adult female rabbits were harvested and processed to obtain stromal vascular fraction (SVF). The SVF were platted to isolate ADSC. Before urethral injection, cells were labeled with DiI marker. The urethra wall was injected with 1 × 10⁷ autologous cells or saline (sham). The urethra was harvested at 2, 4, and 8 weeks to identify DiI-labeled cells.

Results

At 2 and 4 weeks, the ADSCs create a nodule localized in the urethral sub-mucosa. At 8 weeks, the ADSCs spread and integrated with the urethra wall from the initial injection site.

Conclusion

This is the first study to demonstrate a successful autologous ADSCs transplantation. It confirms that ADSCs can survive and integrate within the urethral wall.

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Title: Autologous transplantation of adult adipose derived stem cells into rabbit urethral wall
Authors: Fernando Gonçalves Almeida
Yuri Tulio Dantas Nobre
Katia R. Leite
Homero Bruschini
Publication date: 01-06-2010
Publisher: Springer-Verlag
Published in: International Urogynecology Journal / Issue 6/2010
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-009-1090-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Autologous transplantation of adult adipose derived stem cells into rabbit urethral wall

Abstract

Introduction and hypothesis

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Introduction and hypothesis

Methods

Results

Conclusion

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