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01-05-2009 | Original Article

Estrogen replacement avoids the decrease of bladder innervations in ovariectomized adult virgin rats: in vivo stereological study

Authors: Rogerio de Fraga, Paulo Palma, Miriam Dambros, Cassio L. Z. Riccetto, Carlos Mandarim-de-Lacerda, Ricardo Miyaoka

Published in: International Urogynecology Journal | Issue 5/2009

Abstract

Introduction and hypothesis

The authors quantified the nerve fibers in the bladder wall of ovariectomized rats with and without estradiol replacement.

Methods

This study was conducted on 40 Wistar rats (3 months old). Group 1: remained intact; Group 2: underwent bilateral ovariectomy, and after 30 days was started on subcutaneous sesame oil replacement (0.2 ml per day) for 90 days; Group 3: sham-operated, and after 30 days was started on subcutaneous sesame oil replacement (0.2 ml per day) for 90 days; Group 4: bilateral ovariectomy, and after 30 days was started on subcutaneous injection of 17β-estradiol (10 μg/kg body weight) for 90 days. S-100 was used to stain nerves myelinized fibers on paraffin rat bladder sections. The G-50 grid system was used to quantitatively analyze the fibers.

Conclusion

Long-term estrogen deprivation caused significant changes in bladder innervations, which can be characterized by a decreased number of nerve fibers by 65% (p < 0.001).

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Title: Estrogen replacement avoids the decrease of bladder innervations in ovariectomized adult virgin rats: in vivo stereological study
Authors: Rogerio de Fraga
Paulo Palma
Miriam Dambros
Cassio L. Z. Riccetto
Carlos Mandarim-de-Lacerda
Ricardo Miyaoka
Publication date: 01-05-2009
Publisher: Springer-Verlag
Published in: International Urogynecology Journal / Issue 5/2009
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-009-0811-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Estrogen replacement avoids the decrease of bladder innervations in ovariectomized adult virgin rats: in vivo stereological study

Abstract

Introduction and hypothesis

Methods

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction and hypothesis

Methods

Conclusion

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