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Published in: Knee Surgery, Sports Traumatology, Arthroscopy 8/2013

01-08-2013 | Experimental Study

Increased matrix metalloprotease-3 gene expression in ruptured rotator cuff tendons is associated with postoperative tendon retear

Authors: Masafumi Gotoh, Yasuhiro Mitsui, Hideaki Shibata, Tetsu Yamada, Isao Shirachi, Kenjiro Nakama, Takahiro Okawa, Fujio Higuchi, Kensei Nagata

Published in: Knee Surgery, Sports Traumatology, Arthroscopy | Issue 8/2013

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Abstract

Purpose

The role of matrix metalloproteases (MMPs) in ruptured rotator cuff tendons remains unknown. This study aimed to investigate the gene expression of MMPs in ruptured rotator cuff tendons and to compare their expression levels between patients with and without postoperative tendon retear.

Methods

Twenty-four patients (a median age of 61 years: interquartile range, 55–66 years) with full-thickness rotator cuff tears were examined in this study. The marginal site of the ruptured tendon was harvested during surgery. The mRNA expression levels of collagen types I and III, MMP-1, MMP-3, MMP-7, MMP-9, MMP-13, tissue inhibitor of MMP (TIMP)-1, and TIMP-2 were analysed by real-time reverse transcription polymerase chain reaction. Postoperative retear was evaluated by magnetic resonance imaging at a minimum of 1 year following surgery.

Results

The mRNA expression levels of MMP-3 and TIMP-1 in ruptured rotator cuff tendons were significantly increased in patients with postoperative retear (n = 6), compared with patients without retear (n = 18) (P = 0.04). For collagens, MMP-1, MMP-7, MMP-9, MMP-13, and TIMP-2, there were no significant differences in the mRNA expression levels in ruptured tendons between patients with and without retear.

Conclusions

These results suggest that, in addition to up-regulation of TIMP-1 gene expression, increased MMP-3 gene expression in ruptured rotator cuff tendons is associated with postoperative tendon retear. Thus, drug therapy specifically targeting MMP-3 after rotator cuff repair should be considered in the future.
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Metadata
Title
Increased matrix metalloprotease-3 gene expression in ruptured rotator cuff tendons is associated with postoperative tendon retear
Authors
Masafumi Gotoh
Yasuhiro Mitsui
Hideaki Shibata
Tetsu Yamada
Isao Shirachi
Kenjiro Nakama
Takahiro Okawa
Fujio Higuchi
Kensei Nagata
Publication date
01-08-2013
Publisher
Springer Berlin Heidelberg
Published in
Knee Surgery, Sports Traumatology, Arthroscopy / Issue 8/2013
Print ISSN: 0942-2056
Electronic ISSN: 1433-7347
DOI
https://doi.org/10.1007/s00167-012-2209-x

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