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Intensive Care Medicine
Published in: Intensive Care Medicine 6/2005

01-06-2005 | Experimental

Protective effect of β-glucan on lung injury after cecal ligation and puncture in rats

Authors: Hulya Babayigit, Can Kucuk, Erdogan Sozuer, Cevad Yazici, Kader Kose, Hulya Akgun

Published in: Intensive Care Medicine | Issue 6/2005

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Abstract

Objective

Understanding the biological mediators involved in the complex inflammatory response of sepsis and acute lung injury offers the possibility of future investigations targeting treatment based on these mediators. This study investigated whether macrophage activator β-glucan has a protective effect on acute lung injury in an experimental model of sepsis.

Design and setting

Experimental study in an experimental research center.

Materials

30 rats randomized into three groups (sham, sepsis, and β-glucan).

Interventions

Cecal ligation and puncture were performed in the β-glucan and sepsis groups. The β-glucan group was given a single intraperitoneal dose of β-glucan (4 mg/kg) following cecal ligation.

Measurements and results

Rats treated with β-glucan had fewer circulating neutrophils, more blood monocytes, and higher serum interleukin 6 levels than septic animals. The percentages of neutrophils and lymphocytes from the bronchoalveolar lavage fluid and the myeloperoxidase activity measured in the lung tissue were lower in the β-glucan group than in the sepsis group. Less alveolar hemorrhage and neutrophil infiltration were observed in lungs from animals in the β-glucan group in the septic groups.

Conclusions

In this rat model of intra-abdominal sepsis β-glucan treatment partially protected against secondary lung injury, decreased lung hemorrhages, and lung neutrophilia. These results suggest that β-glucan protects against sepsis-associated lung damage.
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Metadata
Title
Protective effect of β-glucan on lung injury after cecal ligation and puncture in rats
Authors
Hulya Babayigit
Can Kucuk
Erdogan Sozuer
Cevad Yazici
Kader Kose
Hulya Akgun
Publication date
01-06-2005
Publisher
Springer-Verlag
Published in
Intensive Care Medicine / Issue 6/2005
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI
https://doi.org/10.1007/s00134-005-2629-x

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