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Intensive Care Medicine
Published in: Intensive Care Medicine 3/2005

01-03-2005 | Experimental

Subcutaneous gas tensions closely track ileal mucosal gas tensions in a model of endotoxaemia without anaerobism

Authors: Bala Venkatesh, Thomas J. Morgan, Jonathan Hall, Zolton Endre, Desley Willgoss

Published in: Intensive Care Medicine | Issue 3/2005

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Abstract

Objective

Few comparative data exist on the responses of the subcutaneous and splanchnic circulations to evolving endotoxic shock. We therefore compared continuous subcutaneous pO2 (pO2sc) and pCO2 (pCO2sc) with simultaneous continuous gut luminal pCO2 (pCO2gi) in an animal model of endotoxaemia and examined whether changes in gas tensions track tissue energy charge (EC).

Design

Prospective observational study.

Subjects

Fourteen anaesthetized rats, 7 controls and 7 experimental.

Interventions

Controls were injected with saline, the experimental group with 20 mg/kg Klebsiella endotoxin. pCO2sc, pO2sc, and pCO2gi were measured continuously. Plasma lactate concentrations were measured at defined periods during the study. After 2 h ileal segments were snap frozen and assayed for tissue EC.

Measurements and results

Endotoxaemia resulted in a significant decrease in mean arterial blood pressure (132±9 to 71±20 mmHg) and pO2sc (71±23 to 33±22 torr) and a significant increase in pCO2gi (58±10 to 90±20 torr) and pCO2sc (56±6 to 81±25 torr). During endotoxaemia pCO2gi was directly correlated with pCO2sc (R 2=0.5) and inversely correlated with pO2sc (R 2=0.63). Plasma lactate concentrations were significantly elevated from baseline in the endotoxin limb. The mean EC was not significantly different in the two groups.

Conclusions

Both subcutaneous tissue gas tensions and intestinal luminal carbon dioxide tensions are rapidly responsive during evolving hypodynamic endotoxic shock. Alterations in tissue gas tensions were not associated with dysoxia.
Appendix
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Metadata
Title
Subcutaneous gas tensions closely track ileal mucosal gas tensions in a model of endotoxaemia without anaerobism
Authors
Bala Venkatesh
Thomas J. Morgan
Jonathan Hall
Zolton Endre
Desley Willgoss
Publication date
01-03-2005
Publisher
Springer-Verlag
Published in
Intensive Care Medicine / Issue 3/2005
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI
https://doi.org/10.1007/s00134-005-2558-8

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