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01-06-2004 | Experimental

Hyperbaric oxygen therapy prevents vascular derangement during zymosan-induced multiple-organ-failure syndrome

Authors: Francesco Imperatore, Salvatore Cuzzocrea, Carlo Luongo, Giovanni Liguori, Antonella Scafuro, Antonella De Angelis, Francesco Rossi, Achille P. Caputi, Amelia Filippelli

Published in: Intensive Care Medicine | Issue 6/2004

Abstract

Objective

This study investigated the effects of hyperbaric oxygen (HBO) therapy on the cardiovascular alteration (e.g. mean arterial pressure, vascular reactivity of thoracic aorta rings changes) caused by zymosan in rats.

Design

Rats.

Setting

University research laboratory.

Intervention and measurements

We investigated the effects of HBO therapy (2 ATA at the fourth and eleventh hours after study onset) on the cardiovascular alteration caused by zymosan (500 mg/kg, administered i.p. as a suspension in saline) in rats. Cardiovascular alterations were assessed 18 h after administration of zymosan and/or HBO therapy.

Results

Treatment of rats with HBO therapy attenuated the vasoplegic response to zymosan. In fact, the analysis of arterial pressure curves revealed no signs of vasoplegic shock. The aorta rings of animals treated with zymosan and HBO had a significantly increased contraction to norepinephrine (NE) and endothelin-1 (ET-1) and dilation to acetylcholine (ACh) compared with the zymosan group. The HBO therapy also attenuated the increase of malondialdehyde (MDA) levels caused by zymosan in the aorta. Immunohistochemical analysis for nitrotyrosine and for iNOS revealed positive staining in the aorta from zymosan-treated rats. The degree of staining for nitrotyrosine and iNOS was markedly reduced in tissue sections obtained from zymosan-rats treated with HBO therapy.

Conclusion

This study provides the first evidence that HBO therapy attenuates the degree of zymosan-induced cardiovascular derangement in the rat.

Cohen IL (1993) Definition of sepsis and organ failure. The ACCP/SCCM Consensus Conference Committee Report. Chest 103:656–666

Bone RC (1991) The pathogenesis of sepsis. Ann Med Int 115:457–469

Hunt BJ (1998) Endothelial cell activation. Br Med J 316:1328–1329

Damas J, Remacle-Volon G, Bourdon V (1993) Platelet–activating factor and vascular effects of zymosan in rats. Eur J Pharmacol 231:231–236CrossRefPubMed

Mainous M, Patrick T, Rodney D (1991) Studies of route, magnitude and time course of bacterial translocation in a model of systemic inflammation. Arch Surg 126:33–39PubMed

Cuzzocrea S, Filippelli A, Zingarelli B, Falciani M, Caputi AP, Rossi F (1997) Role of nitric oxide in a non-septic shock model induced by zymosan in the rat. Shock 7:351–357PubMed

Asmuth EJU von, Maessen JG, Van Der Linden CJ (1990) Tumor necrosis factor alpha (TNFα) and interleukin 6 in zymosan-induced shock model. Scand J Immunol 32:313–319PubMed

Luongo C, Imperatore F, Cuzzocrea S (1998) Effects of hyperbaric oxygen exposure on zymosan-induced shock model. Crit Care Med 26:1972–1976CrossRefPubMed

Rogatsky GG, Shifrin EG, Mayevsky A (1999) Physiologic and biochemical monitoring during hyperbaric oxygenation: a review. Undersea Hyperb Med 26:111–122PubMed

10.

Larsson A, Engstrom M, Uusijarvi J, Kihlstrom L, Lind F, Mathiesen T (2002) Hyperbaric oxygen treatment of postoperative neurosurgical infections. Neurosurgery. 50:287–295

11.

MacFarlane C, Cronje FJ, Benn CA (2000) Hyperbaric oxygen in trauma and surgical emergencies. J R Army Med Corps 146:185–190PubMed

12.

Kovskin VB, Balashov NV, Makarov FN (1995) Design and reactivity of large arterial walls in monkeys after prolonged hyperbaria. Dokl Akad Nauk 340:559–562PubMed

13.

Luongo C, Imperatore F, Matera MG (1999) Effect of hyperbaric oxygen therapy in experimental subcutaneous and pulmonary infections due to Pseudomonas aeruginosa. Under Hyperb Med 26:21–25

14.

Cuzzocrea S, Persichini T, Dugo L, Colasanti M, Musci G. (2003) Copper induces type II nitric oxide synthase in vivo. Free Radic Biol Med. 34:1253–1262

15.

Snedecor GW, Cochran WG (1976) Statistical methods, 6th edn. The Iowa State University Press, Ames, Iowa, pp 549–561

16.

Stoclet JC, Muller B, Gyorgy K, Andriantsiothaina R, Kleschyov AL (1999) The inducible nitric oxide synthase in vascular and cardiac tissue. Eur J Pharmacol 375:139–155CrossRefPubMed

17.

Thiemermann C (1997) Nitric oxide and septic shock. Gen Pharmacol 29:159–166PubMed

18.

McCord J (1993) Oxygen-derived free radicals. New Horizons 1:70–76PubMed

19.

Szabó C, Thiemermann C (1994) Invited opinion: Role of nitric oxide in haemorrhagic, traumatic and anaphylactic shock and in thermal injury. Shock 2:145–155PubMed

20.

Szabó C (1996) The role of peroxynitrite in the pathophysiology of shock, inflammation and ischemia-reperfusion injury. Shock 6:79–88PubMed

21.

Cuzzocrea S, Zingarelli B, Sautebin L, Rizzo A, Crisafulli C, Campo GM, Costantino G, Calapai G, Nava F, Rosa M di, Caputi AP (1997) Multiple organ failure following zymosan-induced peritonitis is mediated by nitric oxide. Shock 4:268–275

22.

Demling R, Nayak U, Ikegami K, LaLonde C (1994) Comparison between lung and liver peroxidation and mortality after zymosan peritonitis in the rats. Shock 2:222–227PubMed

23.

Cuzzocrea S, Zingarelli B, Calapai G, Nava F, Caputi AP (1997) Zymosan-activated plasma induces paw oedema by nitric oxide and prostaglandin production. Life Sci 60:215–220PubMed

24.

Lancaster JR (1992) Nitric oxide in cells. Am Sci 80:248–255

25.

Rao TS, Currie JL, Shaffer AF, Isakson PC (1994) In vivo characterization of zymosan-induced mouse peritoneal inflammation. J Pharmacol Exp Ther 269:917–925PubMed

26.

Cuzzocrea S, Imperatore F, Costantino G, Luongo C, Mazzon E, Scafuro MA, Mangoni G, Caputi AP, Rossi F, Filippelli A (2000) Role of hyperbaric oxygen exposure in reduction of lipid peroxidation and in multiple organ failure induced by zymosan administration in the rat. Shock 13:197–203PubMed

27.

Teixeira TJ, Williams PG, Hellewell PG (1993) Role of prostaglandins and nitric oxide in acute inflammatory reactions in guinea-pig skin. Br J Pharmacol 110:416–422PubMed

28.

Xia Y, Dawson VL, Dawson TM, Snyder SH, Zweier JL (1996) Nitric oxide synthase generates superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury. Proc Natl Acad Sci USA 93:6770–6774PubMed

29.

Salvemini D, Cuzzocrea S (2002) Oxidative stress in septic shock and disseminated intravascular coagulation. Free Radic Biol Med 33:1173–1185CrossRefPubMed

30.

Cuzzocrea S, Riley DP, Caputi AP, Salvemini D (2001) Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacol Rev 53:135–159PubMed

31.

Villa LM, Salas E, Darley-Usmar M, Radomski MEW, Moncada S (1994) Peroxynitrite induces both vasodilatation and impaired vascular relaxation in the isolated perfused rat heart. Proc Natl Acad Sci USA 91:12383–12387PubMed

32.

Elliott SJ (1996) Peroxynitrite modulates receptor-activated Ca²⁺ signaling in vascular endothelial cells. Am J Physiol 270:L954–L961PubMed

33.

Lahat N, Bitterman H, Yaniv N, Kinarty A, Bitterman N (1995) Exposure to hyperbaric oxygen induces tumor necrosis factor-alpha (TNF alpha) secretion from rat macrophages. Clin Exp Immunol 102:655–659PubMed

34.

Beckman JS (1996) Oxidative damage and tyrosine nitration from peroxynitrite. Chem Res Toxicol 9:836–844CrossRefPubMed

35.

Szabó C, Salzman AL, Ischiropoulos H (1995) Peroxynitrite-mediated oxidation of dihydrorhodamine 123 occurs in early stages of endotoxic and hemorrhagic shock and ischemia-reperfusion injury. FEBS Lett 372:229–232CrossRefPubMed

36.

Pelaia P, Rocco M, Blasi RA de, Spadetta G, Alampi D, Araimo FS, Nicolucci S (1995) Assesssment of lipid peroxidation in hyperbaric oxygen therapy. Protective role of N-acetylcysteine. Minerva Anestesiol 61:133–139PubMed

37.

Buras J. (2000) Basic mechanisms of hyperbaric oxygen in the treatment of ischemia-reperfusion injury. Int Anesthesiol Clin Winter 38:91–109

38.

Balentine JD (1973) Selective vulnerability of the central nervous system to hyperbaric oxygen. Adv Exp Med Biol 37A: 293–298PubMed

39.

Clark JM (1982) Oxygen toxicity. In: Bennett PB, Elliot DH (eds) Physiology and medicine of diving, 3rd edn. Bailliere, Tindal and Cox, London, pp 200–238

40.

Mayevsky A (1984) Invited review: brain oxygen toxicity. In: Bachrach AJ, Matzen MM (eds) Undersea physiology, vol 8. Undersea Medical Society, Bethesda, Maryland, pp 69–89

41.

Piantadosi CA, Tatro LG (1990) Regional H₂O₂ concentration in rat brain after hyperoxic convulsions. J Appl Physiol 69:1761–1766

Title: Hyperbaric oxygen therapy prevents vascular derangement during zymosan-induced multiple-organ-failure syndrome
Authors: Francesco Imperatore
Salvatore Cuzzocrea
Carlo Luongo
Giovanni Liguori
Antonella Scafuro
Antonella De Angelis
Francesco Rossi
Achille P. Caputi
Amelia Filippelli
Publication date: 01-06-2004
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 6/2004
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-003-2138-8

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Hyperbaric oxygen therapy prevents vascular derangement during zymosan-induced multiple-organ-failure syndrome

Abstract

Objective

Design

Setting

Intervention and measurements

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Objective

Design

Setting

Intervention and measurements

Results

Conclusion

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