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Diabetologia
Published in: Diabetologia 10/2006

01-10-2006 | Article

Stimulation of cAMP signalling allows isolation of clonal pancreatic precursor cells from adult mouse pancreas

Authors: T. Yamamoto, E. Yamato, H. Taniguchi, M. Shimoda, F. Tashiro, M. Hosoi, T. Sato, S. Fujii, J.-I. Miyazaki

Published in: Diabetologia | Issue 10/2006

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Abstract

Aims/hypothesis

Duct cells of the pancreas are thought to include latent progenitors of islet endocrine cells that can be induced to differentiate by appropriate morphogens. Here we developed a method for isolating pancreatic ductal epithelial cells from adult mice that overcomes the shortcomings of previous methods.

Materials and methods

Pancreatic ductal cells were grown in serum-free DMEM/F12 medium in the presence of cholera toxin or 8-bromo-cyclic adenosine monophosphate, which is known to be an intracellular cAMP generator. Single cell cloning was performed by limiting dilution in serum-free medium.

Results

The isolated clonal cells expressed high levels of cytokeratin and Ipf1 (formerly known as Pdx-1). Adenovirus-mediated expression of ngn3 (also known as Neurog3) and Ptf1a in these cells induced expression of insulin and somatostatin, and of carboxypeptidase A, respectively. Furthermore, albumin production was induced by dexamethasone or by long-term culture in serum-containing medium.

Conclusions/interpretation

Stimulation of the cAMP-dependent signalling allowed us to isolate clonal pancreatic ductal cells from adult mice. These cells are able to partially differentiate into endocrine cells, exocrine cells and hepatocyte-like cells and are therefore considered to have the characteristics of endodermal progenitor cells.
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Metadata
Title
Stimulation of cAMP signalling allows isolation of clonal pancreatic precursor cells from adult mouse pancreas
Authors
T. Yamamoto
E. Yamato
H. Taniguchi
M. Shimoda
F. Tashiro
M. Hosoi
T. Sato
S. Fujii
J.-I. Miyazaki
Publication date
01-10-2006
Publisher
Springer-Verlag
Published in
Diabetologia / Issue 10/2006
Print ISSN: 0012-186X
Electronic ISSN: 1432-0428
DOI
https://doi.org/10.1007/s00125-006-0372-7

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