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Diabetologia

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01-06-2004 | Article

Early vitamin E supplementation attenuates diabetes-associated vascular dysfunction and the rise in protein kinase C-β in mesenteric artery and ameliorates wall stiffness in femoral artery of Wistar rats

Authors: S. J. Wigg, M. Tare, J. Forbes, M. E. Cooper, M. C. Thomas, H. A. Coleman, H. C. Parkington, R. C. O’Brien

Published in: Diabetologia | Issue 6/2004

Abstract

Aims/hypothesis

The impact of early vitamin E supplementation on vascular function in diabetes remains unresolved. Therefore, we examined the effects of vitamin E on functional and structural parameters and on chemical markers that are disturbed in diabetes in mesenteric and femoral arteries.

Methods

Segments of both arteries, taken from control and 8-week-old streptozotocin diabetic Wistar rats that were treated or not with vitamin E, were mounted on wire and pressure myographs, after which endothelium-dependent and -independent vasodilation was assessed. Passive mechanical wall properties and the localisation and levels of protein kinase C (PKC)-β₂ and AGE were evaluated in these vessels.

Results

Vitamin E supplementation was associated with improved endothelium-dependent and -independent vasodilatation in mesenteric arteries from diabetic rats. Impaired endothelium-dependent vasodilatation in diabetic mesenteric vessels was associated with PKC-β₂ up-regulation and this was prevented by vitamin E supplementation. Increased AGE accumulation and plasma isoprostane levels in diabetic rats were not changed by vitamin E. In the femoral artery, vitamin E supplementation had no effect on endothelium-dependent or -independent vasodilatation, but did prevent the wall stiffening associated with diabetes.

Conclusions/interpretation

Early vitamin E supplementation has a beneficial effect on diabetes-induced endothelial dysfunction in resistance arteries. This benefit may arise from a direct effect on smooth muscle function, as a result of inhibition of the PKC-β₂ isoform by vitamin E.

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Title: Early vitamin E supplementation attenuates diabetes-associated vascular dysfunction and the rise in protein kinase C-β in mesenteric artery and ameliorates wall stiffness in femoral artery of Wistar rats
Authors: S. J. Wigg
M. Tare
J. Forbes
M. E. Cooper
M. C. Thomas
H. A. Coleman
H. C. Parkington
R. C. O’Brien
Publication date: 01-06-2004
Publisher: Springer-Verlag
Published in: Diabetologia / Issue 6/2004
Print ISSN: 0012-186X
Electronic ISSN: 1432-0428
DOI: https://doi.org/10.1007/s00125-004-1411-x

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Methods

Results

Conclusions/interpretation

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