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Strahlentherapie und Onkologie

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04-08-2022 | Biomarkers | Original Article

Linear dose response of acrocentric chromosome associations to gamma irradiation in human lymphocytes

Authors: Ravindra M Samarth, Puneet Gandhi, Nabo Kumar Chaudhury

Published in: Strahlentherapie und Onkologie | Issue 2/2023

Abstract

Purpose

The frequency of acrocentric chromosome associations (ACA) was studied to determine the possible dose–response relationship of gamma irradiation in human lymphocytes.

Methods

Peripheral blood collected from three healthy donors was irradiated with 0, 0.1, 0.25, 0.5, 0.75, 1, 2, 3, 4, and 5 Gy of gamma radiation. Chromosomal preparations were made after 48 h of culture as per standard guidelines. The experiment was repeated three times, with a different donor each time.

Results

The ACA frequency in irradiated lymphocytes increased with radiation dose. The D–G type of association was most prominent and showed a significant dose-dependent increase in frequency. The dose response of ACA frequency to radiation was found to be linear: ACA frequency = 0.2923 (±0.0276) + 0.1846 (±0.0307) × D (correlation coefficient r = 0.9442). As expected, dicentric chromosome (DC) frequencies followed the linear quadratic fit model, with DC frequency = 0.0015 (±0.0013) + 0.0220 (±0.0059) × D + 0.0215 (±0.0018) × D^2 (correlation coefficient r = 0.9982). A correlation curve was prepared for ACA frequency versus DC frequency, resulting in the regression equation y = 1.130x + 0.4051 (R² = 0.7408; p = 0.0014).

Conclusion

Our results showed an increase in ACA frequency in irradiated lymphocytes with an increase in radiation dose; thus, ACA may serve as a candidate cytogenetic biomarker for radiation biodosimetry.

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Title: Linear dose response of acrocentric chromosome associations to gamma irradiation in human lymphocytes
Authors: Ravindra M Samarth
Puneet Gandhi
Nabo Kumar Chaudhury
Publication date: 04-08-2022
Publisher: Springer Berlin Heidelberg
Keyword: Biomarkers
Published in: Strahlentherapie und Onkologie / Issue 2/2023
Print ISSN: 0179-7158
Electronic ISSN: 1439-099X
DOI: https://doi.org/10.1007/s00066-022-01978-3

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Linear dose response of acrocentric chromosome associations to gamma irradiation in human lymphocytes

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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