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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

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01-12-2012 | Original article

Gene analysis of signal transduction factors and transcription factors in periodontal ligament cells following application of dynamic strain

Authors: B. Deschner, B. Rath, A. Jäger, J. Deschner, B. Denecke, S. Memmert, W. Götz

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Issue 6/2012

Abstract

Objective

Orthodontic treatment is usually associated with the application of forces to teeth and periodontium. Instrumental in transmitting these forces are the cells of the periodontal ligament (PDL). In the present study, we used an established strain model to investigate the potential role of biophysical stimulation in modulating the gene expression pattern of these PDL cells.

Materials and methods

PDL cells derived from non-carious and periodontally healthy teeth of six patients were grown on culture plates coated with collagen type I. Upon completion of culture, dynamic strain was applied to the cells for 24 h, using 3% of tensile force and a frequency of 0.05 Hz. This loading protocol for biomechanical stimulation was followed by extracting the RNA from the cells and using a RT² PCR array® for analysis.

Results

Compared to non-stimulated control cells, this analysis revealed the induction of several factors (e.g., RELA, IRF1, MAX, MYC, CDKN1B, BCL2, BCL2A1) known to influence tissue homeostasis by contributing essentially to cell proliferation, cell differentiation, and the inhibition of apoptosis.

Conclusion

This study demonstrates that the biomechanical stimulation of PDL cells is an important factor in periodontal tissue homeostasis.

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Title: Gene analysis of signal transduction factors and transcription factors in periodontal ligament cells following application of dynamic strain
Authors: B. Deschner
B. Rath
A. Jäger
J. Deschner
B. Denecke
S. Memmert
W. Götz
Publication date: 01-12-2012
Publisher: Springer-Verlag
Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Issue 6/2012
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-012-0104-1

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Gene analysis of signal transduction factors and transcription factors in periodontal ligament cells following application of dynamic strain

Abstract

Objective

Materials and methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusion

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