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01-04-2018 | Original Research Paper

Methylation of cytokines gene promoters in IL-1β-treated human intestinal epithelial cells

Authors: Fabio Caradonna, Ilenia Cruciata, Ilaria Schifano, Chiara La Rosa, Flores Naselli, Roberto Chiarelli, Anna Perrone, Carla Gentile

Published in: Inflammation Research | Issue 4/2018

Abstract

Objective and design

Epigenetic regulation is important in the activation of inflammatory cells. In the present study, we evaluated if DNA-methylation variations are involved in Interleukin-1β (IL-1β)-induced intestinal epithelial cells activation.

Materials and methods

Differentiated Caco-2 cells were exposed to IL-1β or to 5-azadeoxycytidine (5-azadC) for 24 or 48 h. Genome-wide methylation status was evaluated, while DNA methylation status at the promoter region of the gene encoding interleukin-6, 8 and 10 (IL-6, 8 and 10) was estimated. The levels of the corresponding gene products as well as DNA methyltransferases (DNMTs) quantity were assessed.

Results

IL-1β decreased genomic methylation of human intestinal epithelial cells and induced demethylation at cg-specific sites at the promoter of pro-inflammatory genes IL6 and IL8; conversely it did not change the methylation of the IL10 promoter. IL-1β also increased the release of IL-6 and IL-8 but did not change the IL-10 expression. Finally, cell exposure to IL-1β decreased the DNMT3b expression, increased DNMT3a and was not able to change DNMT1 expression.

Conclusions

Our results suggest a potential role of IL-1β as modulator of DNA methylation in activated differentiated Caco-2 cell line.

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Title: Methylation of cytokines gene promoters in IL-1β-treated human intestinal epithelial cells
Authors: Fabio Caradonna
Ilenia Cruciata
Ilaria Schifano
Chiara La Rosa
Flores Naselli
Roberto Chiarelli
Anna Perrone
Carla Gentile
Publication date: 01-04-2018
Publisher: Springer International Publishing
Published in: Inflammation Research / Issue 4/2018
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-017-1124-5

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Methylation of cytokines gene promoters in IL-1β-treated human intestinal epithelial cells

Abstract

Objective and design

Materials and methods

Results

Conclusions

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Conclusions

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