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Inflammation Research
Published in: Inflammation Research 10/2017

01-10-2017 | Original Research Paper

Pam2CSK4 and Pam3CSK4 induce iNOS expression via TBK1 and MyD88 molecules in mouse macrophage cell line RAW264.7

Authors: Panthong Kulsantiwong, Matsayapan Pudla, Chanya Srisaowakarn, Jitrada Boondit, Pongsak Utaisincharoen

Published in: Inflammation Research | Issue 10/2017

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Abstract

Objective

The aim of this study was to investigate the involvement of TLR adaptor molecules, such as TRIF, MyD88, and TBK1 in the induction of iNOS and nitric oxide (NO) production in Pam2CSK4 and Pam3CSK4-treated mouse macrophages.

Method

Mouse macrophage cell line (RAW264.7) was transfected with trif, myd88, and tbk1 siRNAs before stimulated with Pam2CSK4 and Pam3CSK4. The iNOS gene and protein expression were determined by RT-PCR and immunoblotting, respectively. The NO production was determined by Griess reaction assay.

Results

The results showed that the induction of iNOS expression and NO production by Pam2CSK4 and Pam3CSK4 were diminished in tbk1 and myd88-depleted mouse macrophages but not trif-depleted cells.

Conclusion

These results suggested that the TBK1 and MyD88 molecules were essential for the induction of iNOS expression and NO production by both Pam2CSK4 and Pam3CSK4 via TLR2 signaling.
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Metadata
Title
Pam2CSK4 and Pam3CSK4 induce iNOS expression via TBK1 and MyD88 molecules in mouse macrophage cell line RAW264.7
Authors
Panthong Kulsantiwong
Matsayapan Pudla
Chanya Srisaowakarn
Jitrada Boondit
Pongsak Utaisincharoen
Publication date
01-10-2017
Publisher
Springer International Publishing
Published in
Inflammation Research / Issue 10/2017
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-017-1063-1

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