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Published in: Inflammation Research 8/2012

01-08-2012 | Original Research Paper

Chronic aspiration shifts the immune response from adaptive immunity to innate immunity in a murine model of asthma

Authors: Kuei-Ying Su, Anitra D. Thomas, Jui-Chih Chang, Jason H. Leung, Sean M. Lee, Zoie E. Holzknecht, Mary Lou Everett, W. Michael Foster, Monica Kraft, William Parker, R. Duane Davis, Shu S. Lin

Published in: Inflammation Research | Issue 8/2012

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Abstract

Objective and design

The hypothesis that aspiration of gastric fluid drives the anti-ovalbumin response toward a Th2 reaction even in animals not prone to Th2 responses was evaluated.

Subjects

Forty-eight male C57BL/6 mice were used.

Methods

Mice were sensitized and challenged with ovalbumin starting 5 weeks prior to the initiation of weekly aspirations of either gastric fluid or normal saline as a control. Weekly aspiration continued during the course of exposure to ovalbumin.

Treatment

Aspiration consisted of 50 μl of gastric fluid with 50 μl of 0.9 % normal saline used as a control. Antigen exposure consisted of sensitization to ovalbumin via intraperitoneal injection on days 0 and 14 and challenge on day 21 with aerosolized antigen for 30 min.

Results

No evidence of a shift toward a Th2 response as a result of gastric fluid aspiration was seen in the Th1-prone strain utilized, although a profound down-regulation of a broad array of T cell-associated cytokines and chemokines and up-regulation of macrophage-associated markers was observed as a result of aspiration.

Conclusions

These data provide support for the hypothesis that the clinical association between asthma and gastroesophageal reflux disease (GERD) does not involve an exacerbation of asthma by GERD-associated aspiration of gastric fluid, but may cause immune reactions unrelated to the asthma pathology.
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Metadata
Title
Chronic aspiration shifts the immune response from adaptive immunity to innate immunity in a murine model of asthma
Authors
Kuei-Ying Su
Anitra D. Thomas
Jui-Chih Chang
Jason H. Leung
Sean M. Lee
Zoie E. Holzknecht
Mary Lou Everett
W. Michael Foster
Monica Kraft
William Parker
R. Duane Davis
Shu S. Lin
Publication date
01-08-2012
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 8/2012
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-012-0479-x

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