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Inflammation Research
Published in: Inflammation Research 4/2012

01-04-2012 | Original Research Paper

Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages

Authors: Koon-Yeow Leow, Wilson Wen Bin Goh, Chew-Kiat Heng

Published in: Inflammation Research | Issue 4/2012

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Abstract

Objective

To investigate the effect of serum amyloid A1 (SAA1) on global gene expression in macrophages derived from THP-1 monocytes.

Materials and methods

Global genetic expression in THP-1-derived macrophages was determined using Illumina HT-12 microarray chips and the results were validated by real-time PCR. Cytokine levels in cellular supernatant were quantified by ELISA.

Results

In total, 55 genes were upregulated with fold difference greater than two when THP-1-derived macrophages were incubated with SAA1 for 8 h. SAA1 is a strong cytokine inducer with significant upregulation of chemokines CCL1, CCL3, and CCL4 and this was confirmed by both real-time PCR and ELISA quantification. SAA1 also promotes the upregulation of genes involved in phagocytosis, anti-apoptosis, and tissue remodeling.

Conclusions

SAA1 appears to play an important role during the immune response and in chronic inflammatory diseases through the stimulation of genes involved in cytokine production, phagocytosis, and anti-apoptosis.
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Metadata
Title
Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages
Authors
Koon-Yeow Leow
Wilson Wen Bin Goh
Chew-Kiat Heng
Publication date
01-04-2012
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 4/2012
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-011-0424-4

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WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

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