Top

Inflammation Research

Published in:

Open Access 01-01-2012 | Original Research Paper

Seasonal changes in activities of human neutrophils in vitro

Authors: Magdalena Klink, Katarzyna Bednarska, Edyta Blus, Michal Kielbik, Zofia Sulowska

Published in: Inflammation Research | Issue 1/2012

Abstract

Objective and design

We present a retrospective analysis of previously collected blood samples to determine whether the immune response of neutrophils depends on the season i.e., short versus long days, in which blood samples were collected.

Methods

The bactericidal activity and adhesive capacity of neutrophils, the production of reactive oxygen species (ROS), and CD11b/CD18 molecule expression level were investigated. The investigated neutrophils were divided into two groups based on the time of blood collection: the winter season with short days and the summer season with long days.

Results

We found seasonal variation in measurements of all the analyzed functional responses of neutrophils to stimuli. The strongest adhesion, as well as maximum values of ROS production, was presented by neutrophils isolated from the summer group. The highest bactericidal activity of neutrophils was also observed in blood donors from summer group.

Conclusions

The magnitude of the immune functional activity of neutrophils varies with the season of the year and is decreased in winter.

Lipsitch M, Viboud C. Influenza seasonality: lifting the fog. Proc Natl Acad Sci USA. 2009;106:3645–6.PubMedCrossRef

Nelson RJ. Seasonal immune function and sickness responses. Trends Immunol. 2004;25:187–92.PubMedCrossRef

Prendergast BJ, Wynne-Edwards KE, Yellon SM, Nelson RJ. Photorefractoriness of immune function in male Siberian hamsters (Phodopus sungorus). J Neuroendocrinol. 2002;14:318–29.PubMedCrossRef

Yellon SM, Fagoaga OR, Nehlsen-Cannarella SL. Influence of photoperiod on immune cell functions in the male Siberian hamster. Am J Physiol. 1999;276:R97–102.PubMed

Broadbent S. Seasonal changes in haematology, lymphocytes transferrin receptors and intracellular iron in Ironman triathletes and untrained men. Eur J Appl Physiol. 2010. doi:10.1007/s00421-010-1635-z. Published online 07 September.

Maes M, Stevens W, Scharpe S, Bosmans E, De Meyer F, D’Hondt P, Peeters D, Thompson P, Cosyns P, De Clerck L, Bridts C, Neels H, Wauters A, Cooreman W. Seasonal variation in peripheral blood leukocyte subsets and in serum interleukin-6, and soluble interleukin-2 and -6 receptor concentrations in normal volunteers. Experientia. 1994;50:821–9.PubMedCrossRef

Berger J. Chronohaematology. J Appl Biomed. 2004;2:179–85.

Burg ND, Pillinger MH. The neutrophil: function and regulation in innate and humoral immunity. Clin Immunol. 2001;99:7–17.PubMedCrossRef

Kobayashi SD, Voyich JM, DeLeo FR. Regulation of the neutrophil-mediated inflammatory response to infection. Microbes Infect. 2003;5:1337–44.PubMedCrossRef

10.

Lindbom L, Werr J. Integrin-dependent neutrophil migration in extravascular tissue. Semin Immunol. 2002;14:115–21.PubMedCrossRef

11.

Wagner JG, Roth RA. Neutrophil migration mechanisms, with an emphasis on the pulmonary vasculature. Pharmacol Rev. 2000;52:349–74.PubMed

12.

Roos D, van Bruggen R, Meischl C. Oxidative killing of microbes by neutrophils. Microbes Infect. 2003;5:1307–15.PubMedCrossRef

13.

Klink M, Cedzyński M, Swierzko ASt, Tchórzewski H, Sulowska Z. The involvement of nitric oxide donor compounds in the bactericidal activity of human neutrophils in vitro. J Med Microbiol. 2003;52:303–8.PubMedCrossRef

14.

Klink M, Tchorzewski H, Sulowska Z. Oxidative and adhesive responses of human neutrophils to nitrovasodilators in vitro. The role of protein kinases. Mediators Inflamm. 2003;6:345–53.CrossRef

15.

Klink M, Bednarska K, Jastrzembska K, Banasik M, Sulowska Z. Signal transduction pathways affected by nitric oxide donors during neutrophil functional response in vitro. Inflamm Res. 2007;56:82–90.CrossRef

16.

Klink M, Jastrzembska K, Bednarska K, Banasik M, Sulowska Z. Effect of nitric oxide donors on NADPH oxidase signaling pathway in human neutrophils in vitro. Immunobiology. 2009;214:692–702.PubMedCrossRef

17.

Sulowska Z, Majewska E, Klink M, Banasik M, Tchorzewski H. Flow cytometric evaluation of human neutrophils apoptosis during nitric oxide generation in vitro. The role of exogenous antioxidants. Mediators Inflamm. 2005;2:81–8.CrossRef

18.

Bednarska K, Klink M, Sulowska Z. Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro. Mediators Inflamm. 2006; ID19307:1–8.

19.

Bronson FH. Are humans seasonally photoperiodic? J Biol Rhythm. 2004;19:180–92.CrossRef

20.

Pawlak J, Majewski P, Markowska M, Skwarlo-Sonta K. Season- and gender-dependent changes in the immune function of Siberian hamsters (Phodopus sungorus). Neuro Endocrinol Lett. 2005;26:55–60.PubMed

21.

Killestein J, Rep MH, Meilof JF, Ader HJ, Uitdehaag BM, Barkhof F, van Lier RA, Polman CH. Seasonal variation in immune measurements and MRI markers of disease activity in MS. Neurology. 2002;58:1077–80.PubMed

22.

Myrianthefs P, Karatzas S, Venetsanou K, Grouzi E, Evagelopoulou P, Boutzouka E, Fildissis G, Spiliotopoulou I, Baltopoulos G. Seasonal variation in whole blood cytokine production after LPS stimulation in normal individuals. Cytokine. 2003;24:286–92.PubMedCrossRef

23.

Dowell SF. Seasonal variation in host susceptibility and cycles of certain infectious diseases. Emerg Infect Dis. 2001;7:369–74.PubMed

24.

Recchioni R, Marcheselli F, Moroni F, Gaspar R, Damjanovich S, Pieri C. Melatonin increases the intensity of respiratory burst and prevents L-selectin shedding in human neutrophils in vitro. Biochem Biophys Res Commun. 1998;252:20–4.PubMedCrossRef

25.

Nelson RJ, Drazen DL. Melatonin mediates seasonal changes in immune function. Ann NY Acad Sci. 2000;917:404–15.PubMedCrossRef

26.

Szczepanik M. Melatonin and its influence on immune system. J Physiol Pharmacol. 2007;58(Suppl 6):115–24.PubMed

27.

Silva SO, Rodrigues MR, Ximenes VF, Bueno-da-Silva AEB, Amarante-Mendes GP, Campa A. Neutrophils as a specific target for melatonin and kynuramines: effects on cytokine release. J Neuroimmunol. 2004;156:146–52.PubMedCrossRef

28.

Carrillo-Vico A, Guerrero JM, Lardone PJ, Reiter RJ. A review of the multiple actions of melatonin on the immune system. Endocrine. 2005;27:189–200.PubMedCrossRef

29.

Carrillo-Vico A, Calvo JR, Abreu P, Lardone PJ, Garcia-Maurino S, Reiter RJ, Guerrero JM. Evidence of melatonin synthesis by human lymphocytes and its physiological significance: possible role as intracrine, autocrine, and/or paracrine substance. FASEB J. 2004;18:537–9.PubMed

30.

Coghill R, Baghurst R. Inhibition of melatonin synthesis in human peripheral blood lymphocytes by EMF: a mechanism of infection? In: Ayrapetyan SN, Markov MS, editors. Bioelectromagnetics. Dordrecht: Springer; 2006. p. 399–403.

31.

Pieri C, Recchioni R, Moroni F, Marcheselli F, Marra M, Marinoni S, Di Primio R. Melatonin regulates the respiratory burst of human neutrophils and their depolarization. J Pineal Res. 1998;24:43–9.PubMedCrossRef

32.

Silva SO, Carvalho SR, Ximenes VF, Okada SS, Campa A. Melatonin and its kynurenin-like oxidation products affect the microbicidal activity of neutrophils. Microbes Infect. 2006;8:420–5.PubMedCrossRef

Title: Seasonal changes in activities of human neutrophils in vitro
Authors: Magdalena Klink
Katarzyna Bednarska
Edyta Blus
Michal Kielbik
Zofia Sulowska
Publication date: 01-01-2012
Publisher: SP Birkhäuser Verlag Basel
Published in: Inflammation Research / Issue 1/2012
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-011-0382-x

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Seasonal changes in activities of human neutrophils in vitro

Abstract

Objective and design

Methods

Results

Conclusions

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objective and design

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2012

The effects of leflunomide on CD4+CD25+Foxp3+ T regulatory cells in mice receiving allogeneic bone marrow transplantation

Mast cells cultured from IL-3-treated mice show impaired responses to bacterial antigen stimulation

Nanomedicine: evolutionary and revolutionary developments in the treatment of certain inflammatory diseases

C-peptide reduces pro-inflammatory cytokine secretion in LPS-stimulated U937 monocytes in condition of hyperglycemia

Curcumin inhibits ox-LDL-induced MCP-1 expression by suppressing the p38MAPK and NF-κB pathways in rat vascular smooth muscle cells

Di-(2-ethylhexyl) phthalate induces production of inflammatory molecules in human macrophages

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page