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Inflammation Research
Published in: Inflammation Research 9/2010

01-09-2010 | Original Research Paper

Protective effect of gastrin-releasing peptide receptor antagonist in carrageenan-induced pleural inflammation in rats

Authors: Fabricia Petronilho, Bruna de Souza, Francieli Vuolo, César A. F. Benetton, Emilio L. Streck, Rafael Roesler, Gilberto Schwartsmann, Felipe Dal-Pizzol

Published in: Inflammation Research | Issue 9/2010

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Abstract

Objective

We report the effects of the gastrin-releasing peptide (GRP) receptor antagonist RC-3095 in an acute inflammation model induced by carrageenan.

Methods

Male Wistar rats received saline or saline containing 2% λ-carrageenan into the pleural cavity, with some also receiving RC-3095 3 mg/kg subcutaneously, immediately after surgery. Four hours later, the rats were killed and pleural exudate was obtained for evaluation of total cell count, lactate dehydrogenase activity, total protein, cytokines analysis and nitrite/nitrate concentrations; myeloperoxidase (MPO) activity and oxidative stress were evaluated in the lung.

Results

RC-3095 exhibited pronounced anti-inflammatory actions by inhibition of leukocyte influx and blockade of MPO, nitrite/nitrate and cytokine levels. Moreover, the results showed that RC-3095 elicits action against oxidative damage in lipids and proteins, as well as increasing cell viability.

Conclusion

The present findings suggest that GRP plays a role in acute inflammation that can be related with the reduction of oxidative damage and that it could be effective in therapeutic applications.
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Metadata
Title
Protective effect of gastrin-releasing peptide receptor antagonist in carrageenan-induced pleural inflammation in rats
Authors
Fabricia Petronilho
Bruna de Souza
Francieli Vuolo
César A. F. Benetton
Emilio L. Streck
Rafael Roesler
Gilberto Schwartsmann
Felipe Dal-Pizzol
Publication date
01-09-2010
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 9/2010
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-010-0190-8

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