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Inflammation Research
Published in: Inflammation Research 9/2009

01-09-2009 | Original Research Paper

Progesterone exerts neuroprotective effects by inhibiting inflammatory response after stroke

Authors: Chao Jiang, Jianping Wang, Xin Li, Chunling Liu, Ningning Chen, Yujin Hao

Published in: Inflammation Research | Issue 9/2009

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Abstract

Objective and design

We evaluated the inhibitory effects of progesterone (PROG) on inflammatory response and its influence on the structure of blood–brain barrier in a permanent model of stroke.

Material

One hundred and twenty adult male Sprague-Dawley rats were used in this study.

Treatments

PROG was dissolved in 22.5% 2-hydroxypropyl-bcyclodextrin and given in a dose of 15 mg/kg by intraperitoneal injection 1 h after permanent occlusion of middle cerebral artery (pMCAO). Additional injections of 15 mg/kg were administered subcutaneously 6, 24, and 48 h after pMCAO.

Methods

The expression of tumor necrosis factor-alpha (TNF-α) and claudin5 was measured by immunohistochemistry and western blot technique. Brain water content was determined by the dry–wet weight method.

Results

TNF-α were increased, but claudin5 were reduced in vehicle-treated rats after pMCAO. PROG-treated rats showed a substantial reduction in the expression of TNF-α compared to vehicle controls. In addition, there was significant increase in the expression of claudin5 in the pMCAO rats treated with PROG compared to vehicle. Examination of the water content of the brain also revealed that administration of PROG significantly attenuated the amount of water compared to vehicle in the ipsilateral hemispheres.

Conclusions

These data indicate that PROG is beneficial in this animal model, and may warrant further test in future clinical trials for human stroke.
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Metadata
Title
Progesterone exerts neuroprotective effects by inhibiting inflammatory response after stroke
Authors
Chao Jiang
Jianping Wang
Xin Li
Chunling Liu
Ningning Chen
Yujin Hao
Publication date
01-09-2009
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 9/2009
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-009-0032-8

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