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Published in: Inflammation Research 9/2009

01-09-2009 | Original Research Paper

Housekeeping gene stability in pristane-induced arthritis and antigen-induced pulmonary inflammation of rats

Authors: Congshan Jiang, Liesu Meng, Wenhua Zhu, Muhammad Shahzad, Xudong Yang, Shemin Lu

Published in: Inflammation Research | Issue 9/2009

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Abstract

Objective and design

Aim of this study is to explore the expression stability of 12 commonly used housekeeping genes in inflammation condition.

Materials and methods

Two rat models, pristane-induced arthritis (PIA) and antigen-induced pulmonary inflammation (AIPI) were established and reverse transcription-PCR was performed from rat spleens and inguinal lymph nodes.

Results

The reference gene expression fluctuated in the immune-associated tissues and 18S rRNA expression from the various inflammatory conditions was the most stably in all examined gene expression. Besides, peptidylprolyl isomerase A and beta-glucuronidase in PIA spleens, beta-actin and GAPDH in PIA inguinal lymph nodes, and peptidylprolyl isomerase A and beta-actin in AIPI spleens, expressed more stably than others. These results were further confirmed by expression study of TNF-alpha in PIA rat inguinal lymph nodes.

Conclusion

We suggest that the stable gene mentioned above or geometric mean of three best references in each experimental condition should be used as the reference and the variable ones should be avoided in similar study designs.
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Metadata
Title
Housekeeping gene stability in pristane-induced arthritis and antigen-induced pulmonary inflammation of rats
Authors
Congshan Jiang
Liesu Meng
Wenhua Zhu
Muhammad Shahzad
Xudong Yang
Shemin Lu
Publication date
01-09-2009
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 9/2009
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-009-0027-5

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