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Acta Neurologica Belgica
Published in: Acta Neurologica Belgica 4/2023

05-04-2023 | Central Nervous System Trauma | Original article

miR-211-5p targeting MMP9 regulates the expressions of AQP4 in traumatic brain injury

Authors: Meng Wang, Xin Yu, Bin Li, Chensong Gao, Yan Chen, Xiaoyang Zhang, Wenling Li, Lijun Yang, Zhenzeng Fan

Published in: Acta Neurologica Belgica | Issue 4/2023

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Abstract

Objective

The abnormal expression of matrix metalloproteinase 9 (MMP9) and Aquaporin 4 (AQP4) closely associates with the traumatic brain injury (TBI) development.

Methods

Here, we investigated the relationship between miR-211-5p and MMP9/AQP4 axis in TBI patients and astrocyte cells. Demographics, clinical features, and cerebrospinal fluid (CSF) samples were collected from traumatic brain injury (TBI) patients (n = 96) and controls (n = 30) for pathological and gene expression analyses. Luciferase activity assay and gene expression analyses were performed to dissect the regulatory mechanism of miR-211-5p on MMP9/AQP4 in human astrocyte cells.

Results

miR-211-5p mRNA was significantly decreased in the CSF of TBI patients, which positively correlated with the expression of both MMP9 and AQP4. miR-211-5p could target MMP9 directly in SVG P12 cells. Overexpression of miR-211-5p decreased the expression of MMP9, on the contrary, knockdown miR-211-5p through inhibitors increased the expression of both MMP9 and AQP4.

Conclusion

miR-211-5p inhibits the MMP9/AQP4 axis in human astrocyte cells, which represents a promising approach for the TBI treatment.
Appendix
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Metadata
Title
miR-211-5p targeting MMP9 regulates the expressions of AQP4 in traumatic brain injury
Authors
Meng Wang
Xin Yu
Bin Li
Chensong Gao
Yan Chen
Xiaoyang Zhang
Wenling Li
Lijun Yang
Zhenzeng Fan
Publication date
05-04-2023
Publisher
Springer International Publishing
Published in
Acta Neurologica Belgica / Issue 4/2023
Print ISSN: 0300-9009
Electronic ISSN: 2240-2993
DOI
https://doi.org/10.1007/s13760-023-02205-1

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