Evaluation of the efficacy of three indigenous strains of entomopathogenic nematodes from Meghalaya, India against mustard sawfly, Athalia lugens proxima Klug (Hymenoptera: Tenthredinidae)

The objective of this study was to evaluate the efficacy of three indigenous strains of entomopathogenic nematodes (EPN) from Meghalaya, India, namely Heterorhabditis indica Poinar, Karunakar and David, Steinernema thermophilum Ganguly and Singh, and Steinernema glaseri (Steiner) against the last instar larva of mustard sawfly, Athalia lugens proxima Klug, a serious pest of mustard and radish in India. The larvae of A. lugens proxima were exposed to 10, 25, 50, 75 and 100 infective juveniles (IJs) concentration of each nematode species in Petri dishes. Percentage larval mortality and nematode reproduction in insect larvae was studied. The sawfly larvae were found to be susceptible to all the three EPNs tested, but the degree of susceptibility to infection varied from among nematode species. Based on LC50 value, H. indica was the most pathogenic species. Nevertheless, S. thermophilum and S. glaseri also showed a high insect mortality. This study also revealed that all the three test nematodes are also able to propagate in the host cadaver and produce first generation infective juveniles. However, H. indica produced significantly more number of IJs per insect larva than the other two nematode species. The progeny production was recorded to be the least in case of S. glaseri. In conclusion, our findings suggest that of the three indigenous EPNs studied, H. indica and S. thermophilum have good potential as biological control agents against mustard sawfly, A. lugens proxima.