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Clinical Orthopaedics and Related Research®

Published in:

01-10-2011 | Symposium: Clinically Relevant Strategies for Treating Cartilage and Meniscal Pathology

Integration of Tissue-engineered Cartilage With Host Cartilage: An In Vitro Model

Authors: John S. Theodoropoulos, MD, J. N. Amritha De Croos, PhD, Sam S. Park, BSc, Robert Pilliar, PhD, Rita A. Kandel, MD

Published in: Clinical Orthopaedics and Related Research® | Issue 10/2011

Abstract

Background

We developed a tissue-engineered biphasic cartilage bone substitute construct which has been shown to integrate with host cartilage and differs from autologous osteochondral transfer in which integration with host cartilage does not occur.

Questions/purposes

(1) Develop a reproducible in vitro model to study the mechanisms regulating tissue-engineered cartilage integration with host cartilage, (2) compare the integrative properties of tissue-engineered cartilage with autologous cartilage and (3) determine if chondrocytes from the in-vitro formed cartilage migrate across the integration site.

Methods

A biphasic construct was placed into host bovine osteochondral explant and cultured for up to 8 weeks (n = 6 at each time point). Autologous osteochondral implants served as controls (n = 6 at each time point). Integration was evaluated histologically, ultrastructurally, biochemically and biomechanically. Chondrocytes used to form cartilage in vitro were labeled with carboxyfluorescein diacetate which allowed evaluation of cell migration into host cartilage.

Results

Histologic assessment demonstrated that tissue-engineered cartilage integrated over time, unlike autologous osteochondral implant controls. Biochemically there was an increase in collagen content of the tissue-engineered implant over time but was well below that for native cartilage. Integration strength increased between 4 and 8 weeks as determined by a pushout test. Fluorescent cells were detected in the host cartilage up to 1.5 mm from the interface demonstrating chondrocyte migration.

Conclusions

Tissue-engineered cartilage demonstrated improved integration over time in contrast to autologous osteochondral implants. Integration extent and strength increased with culture duration. There was chondrocyte migration from tissue-engineered cartilage to host cartilage.

Clinical Relevance

This in vitro integration model will allow study of the mechanism(s) regulating cartilage integration. Understanding this process will facilitate enhancement of cartilage repair strategies for the treatment of chondral injuries.

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Title: Integration of Tissue-engineered Cartilage With Host Cartilage: An In Vitro Model
Authors: John S. Theodoropoulos, MD
J. N. Amritha De Croos, PhD
Sam S. Park, BSc
Robert Pilliar, PhD
Rita A. Kandel, MD
Publication date: 01-10-2011
Publisher: Springer-Verlag
Published in: Clinical Orthopaedics and Related Research® / Issue 10/2011
Print ISSN: 0009-921X
Electronic ISSN: 1528-1132
DOI: https://doi.org/10.1007/s11999-011-1856-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Integration of Tissue-engineered Cartilage With Host Cartilage: An In Vitro Model

Abstract

Background

Questions/purposes

Methods

Results

Conclusions

Clinical Relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Questions/purposes

Methods

Results

Conclusions

Clinical Relevance

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